LncRNA5251通过修改细胞-细胞结节来抑制精子发生
Cong Zhang1,2, Dongxin Lu1,3, Tong Niu1
1Laboratory of Animal Reproductive Physiology and Disease, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, P. R. China.
Biology direct
|June 14, 2023
概括
长非编码RNA 5251 (lncRNA5251) 在男性生育能力中起着至关重要的作用. 调节lncRNA5251通过影响细胞结基因表达,影响精子运动和精子生成.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 细胞生物学 细胞生物学
背景情况:
- 男性不孕症是一个日益严重的全球性问题,长非编码RNAs (lncRNAs) 成为生物过程中的关键调节者.
- LncRNAs已经涉及到各种功能,包括精子生成的复杂过程.
研究的目的:
- 研究lncRNA5251在小鼠精子生成中的特定作用.
- 了解 lncRNA5251 影响男性生殖功能的分子机制.
主要方法:
- 在小鼠丸 (体内) 和精子干细胞 (C18-4细胞,体内) 中使用短毛RNA (shRNA) 调节LncRNA5251的表达.
- 对精子运动,基因表达 (通过基因本体学丰富分析) 和蛋白质水平的影响在两代 (muF0和muF1) 进行了评估.
主要成果:
- 在两代人中,lncRNA5251的过度表达显著降低了精子运动性.
- 抑制了与细胞结合和精子生成相关的 lncRNA5251上调基因.
- 过度表达 lncRNA5251 下调调节的基因,参与精子生成和免疫通路.
- 在体外,lncRNA5251 knockdown 增强了 C18-4 细胞中的细胞结基因和蛋白质表达.
结论:
- 通过细胞结节的调节,LncRNA5251与精子生成有关.
- 这些发现为潜在的治疗策略提供了理论基础,针对 lncRNAs 提高男性生殖能力.
更多相关视频
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
03:17Inheritance of Chromatin Structures
6.3K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.3K
01:41Spermatogenesis
102.7K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.7K
01:23Negative Regulator Molecules
35.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.5K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K