相关实验视频
Updated: Jul 26, 2025

07:37
An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
Published on: June 6, 2025
222
由内源性逆转录病毒K102编码的信封蛋白在类风湿性关节炎中性粒细胞中的表达
Amanda Laine1, Xiaoxing Wang1, Kathryn Ni1
1Division of Rheumatology, Department of Medicine, University of Washington, Seattle, WA 98195, USA.
Microorganisms
|June 15, 2023
概括
类风湿性关节炎患者表现出针对人类内源性逆转录病毒K (HERV-K) 包膜蛋白的自身抗体. 在RA中性粒细胞中,HERV-K102表达升高,导致它们表面可检测的Env.
科学领域:
- 免疫学 免疫学 免疫学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 自身免疫性疾病往往涉及对来自基因组反元素的蛋白质的自身抗体.
- 表观遗传沉默可能不足以防止这些蛋白质的免疫耐受性崩.
- 人体内源逆转录病毒K (HERV-K) 包裹 (Env) 蛋白质是这样一个目标.
研究的目的:
- 为了研究HERV-K表达在类风湿性关节炎 (RA) 中性粒细胞.
- 为了确定哪些HERV-K位点产生Env被患者自身抗体识别.
- 为了确定Env是否存在于RA中性粒细胞的表面.
主要方法:
- 对RA中性粒细胞的RNA测序来分析HERV-K表达.
- 对患者内源性表达Env.的自身抗体识别的分析.
- 使用单克隆抗体在免疫细胞表面对Env的免疫检测.
主要成果:
- 只有HERV-K102和HERV-K108位点显示了Env表达的完整的开放读取框架.
- 与对照组相比,HERV-K102表达在RA中性粒细胞中特别增加.
- 患者自身抗体和单克隆抗Env抗体检测到RA中性粒细胞上的Env.
结论:
- HERV-K102是主要负责产生在RA中性粒细胞表面上可检测的Env的位点.
- 在RA中性粒细胞中HERV-K102表达的增加有助于自身抗体的识别.
- 在中性粒细胞上,HERV-K108表达可能在细胞表面Env中起到较小的作用.
相关概念视频
Retroviruses
12.5K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.5K
T Cell Types and Functions
1.1K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.1K
Rous Sarcoma Virus (RSV) and Cancer
5.2K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.2K
Antigens Involved in Adaptive Immunity
564
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
564
NF-κB-dependent Signaling Pathway
7.5K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.5K
Rheumatic Heart Disease I: Introduction
17
Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...
17

