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综合微生物群和代谢组分析,以评估玉米-大豆食料的固态发酵的影响,使用复合菌株

Yue Li¹, Qinghong Hao¹, Chunhui Duan²

¹College of Life Sciences, Hebei Agricultural University, Baoding 071001, China.

|June 15, 2023

概括

用特定细菌提高蛋白质质量和改变代谢物概况的玉米-大豆-小麦的固态发酵料. 像Pediococcus,Enterococcus和Lactobacillus这样的关键微生物驱动蛋白质降解和氨基酸代谢,改善料的营养价值.

科学领域:

微生物学微生物学
生物化学生物化学
动物营养动物营养

背景情况:

固态发酵 (SSF) 提高了植物性动物料的营养价值.
在料的SSF过程中微生物群落和代谢物生产之间的复杂关系仍然不完全理解.

研究的目的:

为了研究玉米-大豆-小麦 (CSW) 料SSF期间的微生物和代谢变化.
阐明特定微生物种群和代谢物概况之间的相关性.
了解关键细菌系在料发酵中的作用.

主要方法:

用Bacillus licheniformis,Bacillus subtilis和乳酸细菌对CSW粉进行注射.
16S rDNA测序用于微生物社区分析.
针对代谢物识别而进行非向的代谢学分析.
二甲基硫酸盐-多烯胺凝电泳 (SDS-PAGE) 用于蛋白质分析.

主要成果:

三酸可溶性蛋白质的显著增加和甘氨酸和β-甘氨酸的减少.
在发酵料中Pediococcus,Enterococcus和Lactobacillus的占主导地位.
鉴定了699种显著不同的代谢物,其中阿尔金因和プロ林代谢是关键途径.
在Enterococcus/Lactobacillus和特定的二之间,以及在Pediococcus和与营养和免疫相关的代谢物之间,存在正相关性.

结论:

在发酵的CSW料中,Pediococcus,Enterococcus和Lactobacillus对于蛋白质降解,氨基酸代谢和乳酸生产至关重要.
这些微生物有助于改善料的营养质量和潜在的免疫功能.
该研究提供了优化SSF流程的见解,以提高料生产效率.

关键词:

16S rDNA 测序的使用细菌类类类这种细菌是 Bacillus subtilis.料发酵料发酵料发酵料发酵乳酸细菌是一种乳酸细菌.微生物群落中的微生物群落.没有目标的代谢学.

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