在alphaproteobacteriumMagnetospirillum gryphiswaldense中外来磁体基因的功能表达
Ram Prasad Awal1, Christopher T Lefevre2, Dirk Schüler1
1Department of Microbiology, University of Bayreuth , Bayreuth, Germany.
mBio
|June 15, 2023
概括
磁螺 (Magnetospirillum gryphiswaldense) 作为一种多功能宿主,可以表达来自各种细菌的磁体基因. 这项研究促进了磁体体基因集群移植和生物技术应用的工程.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 遗传学 是一个遗传学.
背景情况:
- 磁基因组,在磁带性细菌 (MTB) 中的纳米尺寸的磁性晶体,通过复杂的基因集群 (MGC) 合成.
- 研究来自多种MTB的MGC是具有挑战性的,因为它们无法用于基因操纵.
- 磁体体基因在可处理宿主中的功能表达对于理解它们的生物发生和工程潜力至关重要.
研究的目的:
- 在模型生物Magnetospirillum gryphiswaldense.se.中研究各种MTB中保存磁体基因的功能表达.
- 评估M. gryphiswaldense作为MGCs异质表达的替代宿主的潜力.
- 通过基因集群转移和组装探索磁体组生物矿物化的工程.
主要方法:
- 在M. gryphiswaldense突变体中通过染色体集成对正义磁体组基因的功能表达.
- 补充试验用于评估磁体组生物合成的恢复.
- 转换相关的重组克隆用于组装和转移整个MGCs.
- 相互作用蛋白 (MamB,MamM) 的共同表达,以增强功能补充.
主要成果:
- 来自密切相关的Alphaproteobacteria的单独的ortologs部分恢复了磁体组生物合成.
- 来自远距离相关的Magnetococcia和Deltaproteobacteria的正义词被表达出来,但未能恢复生物合成.
- MamB和MamM的共同表达改善了功能补充.
- 从M.magneticum组装的MGC恢复了删除突变体和M. gryphiswaldense.中的磁铁生物矿物化.
- 来自不同物种的MGCs的共同表达导致了磁分体的过度生产.
结论:
- M. gryphiswaldense 是一个适合功能磁体体基因表达的替代宿主.
- 与转换相关的重组克隆促进了大型MGCs的组装和转移.
- 这种方法使得研究和工程磁体生物生成生物技术应用,包括创建磁石晶体与量身定制的形态.
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