使用Solanum pennellii内进线发现与干旱耐受性相关的番茄候选基因
Herika Paula Pessoa1, Françoise Dalprá Dariva1, Mariane Gonçalves Ferreira Copati1
1Department of Agronomy, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
PloS one
|June 15, 2023
概括
这项研究确定了耐干旱的番茄内进线 (ILs),从幼苗到繁殖阶段保持耐受性. 在精英番茄品种中,还发现了几种改善干旱抵抗力的候选基因.
科学领域:
- 植物遗传学 植物遗传学
- 农作物科学 农作物科学
- 农业生物技术 农业生物技术
背景情况:
- 番茄 (Solanum lycopersicum) 容易受到干旱压力,影响产量和质量.
- 干旱耐受性在所有植物发育阶段对于种植物生存能力至关重要.
- 索拉南的内进线 (IL) 为识别耐旱基因提供了宝贵的资源.
研究的目的:
- 评估Solanum pennellii ILs是否在发芽期间先前被确定为耐旱,在后来的植物和繁殖阶段保持这种特征.
- 在这些IL中确定与干旱耐受性相关的候选基因.
- 为了为干旱耐受性特征的标记器辅助侵入进入精英番茄品种提供基础.
主要方法:
- 在灌良好和干旱压力条件下对IL的表型评估,重点关注形态,生理,水果质量和产量特征.
- 应用多特征指数 (FAI-BLUP) 来根据其与耐旱意识形态的近距离对基因型进行排名.
- 使用番茄IL种群地图来精确确定入侵细分并识别候选基因.
主要成果:
- 在水果产量,水含量,尺寸,花期腐烂发生率和干旱压力下可定位酸度方面观察到显著的基因型变异.
- 五种IL (IL 1-4-18,IL 7-4-1,IL 7-1,IL 7-5-5,和IL 1-2) 在植物/繁殖阶段表现出耐旱性.
- IL 1-4-18和IL 1-2从幼苗到繁殖阶段表现出持续的干旱耐受性.
- 候选基因包括AHG2,At1g55840,PRXIIF,SAP5,REF4-RELATED1,PRXQ,CFS1,LCD,CCD1和SCS.这些基因被确定为候选基因.
结论:
- 特定的Solanum pennellii ILs具有强大的干旱耐受性,在整个番茄生命周期中持续存在.
- 已识别的候选基因为番茄抗旱能力的基因改进提供了目标.
- 这些基因的标记器辅助内进化有望发展适应气候的番茄品种.
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