研究Mycobacterium tuberculosis sufR (rv1460) 在体外和体外表达和免疫性
Lucinda Baatjies1, Ilana C van Rensberg1, Candice Snyders1
1Division of Molecular Biology and Human Genetics, Department of Science and Innovation (DSI)-National Research Foundation (NRF) Centre of Excellence for Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
PloS one
|June 15, 2023
概括
对于Mycobacterium结核病生存至关重要的SufR蛋白在感染个体中显示出低免疫性. 免疫检测没有检测到对SufR的强烈反应,这表明它可能不是结核病诊断或治疗的可靠目标.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 铁对于Mycobacterium结核病 (M.结核病) 的生存和持续性至关重要.
- 硫 (SUF) 操作子是M.结核病中铁-硫 (Fe-S) 生物发生的关键,在铁限制和细胞内生长过程中诱导.
- 了解SufR表达和免疫性对于开发新的结核病 (TB) 诊断和治疗至关重要.
研究的目的:
- 在细胞内M.结核病生长过程中研究单细胞水平的SufR表达.
- 评估不同结核病感染状态的个体中SufR的免疫性.
主要方法:
- 使用mCherry开发了一个光报告系统来监测sufR促进体活动.
- 在体外培养和THP-1巨细胞细胞内生长期间进行表达分析.
- 通过使用全血测试 (WBA) 和淋巴细胞增殖测试 (LPA) 在活跃的结核病,QuantiFERON阳性和QuantiFERON阴性个体中评估免疫反应.
主要成果:
- 在实验室中,mCherry报告员有效地测量了sufR促进体诱导,但在检测抑制方面遇到了困难.
- 巨细胞的细胞内生长显示了M.结核病的一个子集的诱导,但不是统一的.
- 在所有临床组中,SufR在WBA和LPA中引起了较低的免疫反应,没有强大的细胞因子或生长因子的产生.
结论:
- 在细胞内M.结核病感染期间,SufR表达是动态的,在亚群中观察到诱导.
- 在受M.结核病感染的个体中,SufR似乎没有强烈的免疫性.
- 这些发现表明SufR可能不是基于免疫的结核病诊断或治疗的合适目标.
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