对具有与自闭症谱系障碍相关的差异性表达特征的非编码RNA基因进行系统审查
Jon Stott1,2, Thomas Wright3,4, Jannah Holmes1,5
1Child Oriented Mental Health Intervention Collaborative (COMIC), University of York in Collaboration with Leeds and York Partnership NHS Foundation Trust, York, United Kingdom.
PloS one
|June 15, 2023
概括
这次审查发现,自闭症谱系障碍 (ASD) 中较短的非编码RNA (ncRNA) 基因表达差异的证据有限. 虽然一些微RNA (miRNA) 和小核RNA (snoRNA) 基因表现出改变的表达,但结果不一致,需要进一步进行高质量的研究.
科学领域:
- 遗传学 遗传学 是一个
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 非编码RNAs (ncRNAs) 是从DNA转录的功能分子.
- 微RNAs (miRNAs) 是ncRNAs的一个子集,对于神经系统的发育和调节至关重要.
- 对自闭症谱系障碍 (ASD) 中较短的ncRNA基因表达的研究比对miRNA的研究少.
研究的目的:
- 系统地审查ASD个体中较短的ncRNA基因的差异表达.
- 巩固目前关于ASD中ncRNA基因表达的研究,以指导未来的研究.
主要方法:
- 对2000年1月至2022年5月发表的研究进行系统审查.
- 从比较ASD队列与对照组中ncRNA基因表达的研究中提取的数据.
- 包括ncRNA类:miRNA,piRNA,snaR,snRNA,snoRNA,tRNA,vtRNA和Y.RNA. 这些类包括在内.
主要成果:
- 包括48项研究;大多数专注于miRNA.
- 64个miRNA基因在ASD中表现出差异性表达,通常不一致.
- 四个miRNA基因 (miR-106b-5p,miR-155-5p,miR-146a-5p,miR-328-3p) 显示出显著的差异性表达模式.
- 六项研究报告了ASD中差异表达的snoRNA基因;在多项研究中没有报告其他ncRNA基因.
结论:
- 有限,有希望的证据将某些miRNA和新出现的证据将snoRNA基因表达与ASD联系起来.
- 不一致的发现和可变的研究质量需要谨慎解释.
- 对于未来的高质量研究,建议采用标准化的方法和原始数据报告.
相关概念视频
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
02:39lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
01:19Autism Spectrum Disorder
Autism spectrum disorder (ASD) is a neurodevelopmental condition marked by persistent deficits in social communication and interaction alongside restrictive and repetitive behaviors or interests. ASD is sometimes accompanied by intellectual impairment.
These core symptoms manifest differently among individuals, ranging from mild to severe. The disorder's complexity extends beyond its clinical presentation, encompassing a diverse range of biological, cognitive, and sociocultural influences.
These core symptoms manifest differently among individuals, ranging from mild to severe. The disorder's complexity extends beyond its clinical presentation, encompassing a diverse range of biological, cognitive, and sociocultural influences.