相关实验视频

Updated: Jul 26, 2025

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Published on: June 24, 2016

细菌病原体通道中等大小的脂肪酸变成马莱西普罗尔生物合成

Felix Trottmann¹, Jonas Fiedler¹, Keishi Ishida¹

¹Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Beutenbergstraße 11a, 07745 Jena, Germany.

ACS chemical biology

|June 15, 2023

概括

研究人员发现,中链脂肪酸是Burkholderia pseudomallei病原体中毒性因子maleicyprol的关键组成部分. 这一发现发现了一种新的酶,BurM,对毒素组装至关重要,并提供了新的治疗点.

科学领域:

微生物学微生物学
生物化学生物化学
药物发现药物发现药物发现

背景情况:

伯克霍尔德菌 (Burkholderia pseudomallei) (BP) 群的细菌会导致人类和动物的严重感染.
马莱西普罗尔 (maleicyprol) 是一种多基化物代谢物,对于血压高的毒性至关重要,特别是由于其抗生素耐药性.
马莱西普罗的长水链的起源以前是未知的.

研究的目的:

为了阐明maleicyprol中疏水性基链的生物合成起源.
为了确定参与合成这种毒性因子的特定分子和酶.
探索潜在的治疗点,以对抗高血压感染.

主要方法:

发现和描述新型马莱西普罗尔类原体.
参与maleicyprol生物合成的基因的突变分析.
生物化学测定,包括酶活性体内复制.
对聚基酸合成酶 (PKS) 起始单元和与乙载体蛋白 (ACP) 结合的中间体的分析.

主要成果:

确定了中型脂肪酸作为maleicyprol疏水尾巴的来源.
发现了一种新型的辅酶A-独立脂肪酸乙酸酶 (FAAL),命名为BurM,对于脂肪酸招募和激活至关重要.
通过体外研究证明了BurM在PKS原始化和maleicyprol组装中的关键作用.

结论:

中链脂肪酸是maleicyprol的疏水链的PKS启动单元.
BurM是maleicyprol生物合成中的一个关键酶,对于毒素组装至关重要.
针对BurM提供了一个有前途的策略,用于开发针对BP病原体的抗病毒疗法.

更多相关视频

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

Published on: May 15, 2019

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

相关概念视频

Biosynthesis of Lipids

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...

Biosynthesis in Bacteria

Biosynthesis in Bacteria

Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...

Formation of Lipopolysaccharides

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...

Overview of Fatty Acid Metabolism

Overview of Fatty Acid Metabolism

Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...

Amino Acid Biosynthetic Pathways

Amino Acid Biosynthetic Pathways

Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...

Lipid Catabolism

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...