高多重生物活性查揭示了人类和微生物的代谢基因组-GPCRome相互作用
Haiwei Chen1, Connor E Rosen2, Jaime A González-Hernández2
1Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
科学家开发了PRESTO-Salsa,这是一种新的选技术,用于研究代谢物-G蛋白结合受体 (GPCR) 的相互作用. 这种方法从人类代谢物和微生物组中发现了许多新的GPCR激动剂.
科学领域:
- 生物化学
- 药理学
- 微生物学
背景情况:
- 来自各种来源的成千上万的代谢物与G蛋白结合受体 (GPCR) 相互作用.
- 目前的技术限制了这些关键代谢物-GPCR相互作用的探索.
- 了解这些相互作用对于人类的健康和疾病至关重要.
研究的目的:
- 开发一种高通量选技术来检测代谢物与GPCR的相互作用.
- 确定新的内源性,外源性和微生物GPCR激活剂.
- 创建一个微生物组-GPCR相互作用的图谱.
主要方法:
- 开发一种高度多重的选技术 - - PRESTO-Salsa.
- 对1041种与人相关的代谢物进行查,对300多种GPCR进行查.
- 使用435个人类微生物菌株生成微生物组-GPCR相互作用图谱.
主要成果:
- 从人体代谢物和微生物来源鉴定以前未报告的GPCR激动剂.
- 发现微生物组代谢物的跨组织GPCR参与的保存模式.
- 通过 Porphyromonas gingivalis 蛋白酶 gingipain K 激活了 CD97/ADGRE5.
结论:
- PRESTO-Salsa是一种高通量查代谢物-GPCR相互作用的有效技术.
- 发现了多种类型的代谢物-GPCRome相互作用,包括涉及饮食,药物和微生物组的相互作用.
- 这项工作扩大了我们对人类健康中的代谢体-GPCRome相互作用的理解.
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