调查抗菌剂治疗与抗胺素耐药黄金葡萄球菌增加的发生之间可能存在的联系
Andreas F Wendel1, Robin Otchwemah1, Franziska Layer-Nicolaou2
1Institute of Hygiene, Cologne Merheim Medical Centre, University Hospital of Witten/Herdecke, Cologne, Germany; Division of Hygiene and Environmental Medicine, Department of Human Medicine, Faculty of Health, Witten/Herdecke University, Witten, Germany.
概括
黄金葡萄球菌中达普托米辛耐药性可能来自于用聚乙烯比古安化物 (PHMB) 治疗伤口的消毒剂,而不仅仅是抗生素. 这种耐药性与mprF基因突变有关,突出显示了伤口护理中的潜在风险.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
背景情况:
- 德国科隆日益发现抗达普素 (DAP-R) 耐药黄金葡萄球菌. 德国科隆.
- 需要进行分子监测,以了解DAP-R分离物的出现和传播.
研究的目的:
- 调查DAP-R黄金葡萄球菌的出现和传播的原因.
- 确定聚乙烯比古安化物 (PHMB) 在DAP耐药性发展中的作用.
主要方法:
- 从42名患者中采集了75个金黄色细菌分离物 (DAP-R和DAP敏感).
- 使用微稀释来确定达普素 (DAP) 和PHMB的最小抑制度 (MIC).
- 使用PHMB进行全基因组测序和体外选择实验.
主要成果:
- 主要观察到的DAP耐药性是在用杀菌剂 (PHMB) 而不是全身抗生素治疗伤口的患者中.
- 大多数DAP-R分离物显示PHMB的MIC升高;体外实验证实PHMB选择DAP耐药性.
- 在临床和选定的菌株中,DAP耐药性与mprF基因中的多态性有关.
结论:
- 黄金菌中达普托米辛耐药性可以独立于抗生素治疗而发展,并由PHMB选择.
- 用PHMB治疗伤口可能会通过mprF基因的功能获取突变诱导DAP耐药性.
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