相关实验视频
Updated: Jul 26, 2025

10:41
Identifying Amino Acid Overproducers Using Rare-Codon-Rich Markers
Published on: June 24, 2019
8.4K
菌体介导的溶解支持氨基酸辅酶的强生长
Gordon J Pherribo1, Michiko E Taga2
1Department of Plant & Microbial Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
The ISME journal
|June 15, 2023
概括
病毒溶解,而不是分泌或机械溶解,有效释放细胞内营养物质. 这一过程支持了辅助性微生物的生长,这表明病毒通过提供必要的代谢物来塑造微生物群落.
科学领域:
- 微生物学 微生物学
- 微生物生态学 微生物生态学
- 病毒学 病毒学
背景情况:
- 微生物群落中含有辅体,即无法合成必需代谢物的生物.
- 辅助性植物依赖其他物种提供营养,但细胞内代谢物释放的机制尚不清楚.
- 分泌和细胞溶解是代谢物释放的假设途径.
研究的目的:
- 研究生产细菌释放细胞内代谢物的机制.
- 为了确定分泌,机械溶解或病毒溶解是否支持工程性辅食菌的生长.
- 阐明病毒溶解在微生物群落内的代谢物可用性中的作用.
主要方法:
- 用大肠杆菌的工程氨基酸辅食物来测试生长支持.
- 使用无细胞超级生物,机械溶解细胞和生产细菌 (大肠杆菌和细菌杆菌) 的菌体溶解物 (Escherichia coli和 Bacteroides thetaiotaomicron) 来评估代谢物释放.
- 从不同的溶解方法中量化了氨基酸的可用性.
主要成果:
- 无细胞超浮体和机械溶解的细胞提供了最小的氨基酸支持对辅助细胞的生长.
- 菌体溶解物支持大量的辅助性细胞生长,每个溶解的生产细胞支持多达47个辅助性细胞.
- 不同的菌体溶解物释放出不同的氨基酸配置文件,表明不同的营养贡献.
结论:
- 菌体介导的溶解是释放细胞内代谢物的高效机制.
- 病毒溶解对微生物群落的辅助性成员提供必需的营养素有显著的贡献.
- 病毒溶解可能是通过代谢物供应塑造微生物社区结构的主要因素.
相关概念视频
Viral Replication: Lysogenic Cycle
87
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
87
Lysogenic Cycle of Bacteriophages
62.6K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.6K
Lytic Cycle of Bacteriophages
71.1K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
71.1K
Amino Acid Catabolism
59
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
59
Viral Replication: Lytic Cycle
92
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
92
DNA Bacteriophages
62
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
62

