在胚胎基因调节网络中,反回路众多,对这些网络的进化产生稳定影响
Abdull Jesus Massri1, Brennan McDonald1, Gregory A Wray1
1Department of Biology, Duke University, Box 90338, Durham, NC, 27708, USA.
海中的发育基因调节网络 (dGRNs) 高度保存. 尽管有进化差异,但基因表达和反回路的微妙时间差异表明,在这些保存的网络中,相互作用正在演变.
科学领域:
- 发展生物学 发展生物学
- 进化发育生物学 进化发育生物学
- 基因组学就是基因组学.
背景情况:
- 发育基因调节网络 (dGRNs) 对胚胎发育至关重要.
- 海物种Lytechinus variegatus (Lv) 和Strongylocentrotus purpuratus (Sp) 尽管有5000万年的分歧,但共享了一个保存的dGRN.
- 以前的研究表明,Lv和Sp dGRNs之间的早期基因表达时间可能存在差异.
研究的目的:
- 重新分析和比较Lv和Sp的dGRNs,重点关注初始基因表达的精确时间.
- 为了识别和描述两种物种的dGRNs中的反回路.
- 研究关键发育基因中的时间差异 (异时差异) 的进化起源和影响.
主要方法:
- 来自Lv和Sp胚胎的scRNA-seq数据的比较分析.
- 在dGRNs中的基因表达的详细时间概况.
- 基于时间表达模式的调节反回路的推断.
- 基因表达时间在多种海鱼物种中的基因表达基因基因比较.
主要成果:
- 关键细胞命运规范基因的初始表达在两种物种的压缩时间段中发生.
- 之前未被识别的反回路被确定并从暂时纠正的dGRNs中推断出来.
- 在Lv和Sp之间观察到关键调节基因首次表达时间的显著差异.
- 与第三个物种的比较表明,异构物种是独立于血统和进化分支的.
结论:
- 高度保存的dGRNs仍然可以容纳不断演变的相互作用和调节电路.
- 在dGRNs中的反回路可能在缓冲表达时间变化 (异时) 的影响方面发挥作用.
- 进化可塑性存在于基因激活的时间内,即使在深度保存的发育网络中.
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