通过SlMPK1和SlMPK2介导的SlBBX17酸化积极调节番茄中CBF依赖的寒冷耐受性
Jianing Song1, Rui Lin1, Mingjia Tang1
1Department of Horticulture, Zijingang Campus, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
The New phytologist
|June 16, 2023
概括
番茄植物的寒冷耐受性是由SlBBX17增强的,它是一种B盒 (BBX) 蛋白质. 这种蛋白与SlHY5和SlMPK1/2合作,在寒冷压力下促进C重复结合因子 (CBF) 基因表达.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- B-box (BBX) 蛋白质是植物发育和应激反应中的关键转录因子.
- 在番茄寒冷耐受机制中,BBX蛋白的特定作用尚不清楚.
研究的目的:
- 为了研究番茄 (Solanum lycopersicum) 中的SlBBX17蛋白质的功能,研究了耐寒性.
- 阐明SlBBX17调节冷反应的分子机制.
主要方法:
- 使用了反向遗传学,生化分析和分子生物学技术.
- 分析了SlBBX17,SlHY5和SlMPK1/2之间的相互作用.
- 在寒冷压力下评估基因表达和蛋白质稳定性.
主要成果:
- 过度表达SlBBX17增强了耐寒性,而沉默增加了敏感性.
- SlBBX17与 SlHY5 物理相互作用,促进 SlHY5 稳定性和 SlCBF 基因转录.
- 冷激活的SlMPK1和SlMPK2酸化SlBBX17,增强了SlBBX17-SlHY5相互作用.
结论:
- 一个涉及SlMPK1/2,SlBBX17和SlHY5的新型信号通路调节了SlCBF转录,以增强番茄的寒冷耐受性.
- 这项研究为了解涉及多个转录因子的植物冷应激反应提供了一个机制框架.
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