在莉花色的信号中组装JAZ-JAZ和JAZ-NINJA复合体
X Edward Zhou1, Yaguang Zhang2, Jian Yao3
1Department of Structural Biology, Van Andel Institute, Grand Rapids, MI 49503, USA.
Plant communications
|June 16, 2023
概括
斯蒙酸盐 (JAs) 通过控制MYC转录因子来调节植物的应激反应. 这项研究揭示了JAZ和NINJA蛋白质如何动态相互作用以形成抑制器复合体,在组装时稳定.
科学领域:
- 植物分子生物学 植物分子生物学
- 激素信号通路是激素的信号通路.
- 蛋白质与蛋白质的相互作用
背景情况:
- 斯酸盐 (JAs) 是关键的植物激素,调节发育和应激弹性.
- 通过促进JAZ抑制剂的降解,JA激活MYC转录因子.
- 在没有JA的情况下,JAZ蛋白与NINJA一起,与MYC和TPL蛋白形成抑制复合体.
研究的目的:
- 为了描述JAZ-JAZ和JAZ-NINJA在JA抑制器复合体内的相互作用.
- 阐明这些蛋白相互作用的结构动态和稳定机制.
- 提供关于JAZ-NINJA核心结构及其在JA信号传输中的作用的见解.
主要方法:
- 生物化学和生物物理分析.
- 现场定向突变发生的研究.
- 由AlphaFold衍生而来的ColabFold建模.
- 分析蛋白质域动态的分析.
主要成果:
- 描述了JAZ-JAZ和JAZ-NINJA相互作用,产生了高可信度域界面模型.
- JAZ,NINJA和MYC的接口域在孤立时是动态的,但在复杂的组装时逐步稳定.
- 在ZIM图案中表达的JAZ指介绍了相互作用,NINJA调节了JAZ的二分化.
- 接口之外的区域仍然非常动态,很难建模.
结论:
- 这项研究提供了对JA抑制器复合体动态JAZ-NINJA核心的详细结构见解.
- 了解这些相互作用可以进一步了解植物中莉花的信号通路.
- 这些发现突出了复杂形成时内在无序的蛋白质的逐步稳定.
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