聚合物阴离子体及其基于DNA的等离子体复合体作为基因传递载体的稳定性
Mohamed Mashal1, Noha Attia1,2, Santiago Grijalvo3,4
1NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
Drug delivery
|June 16, 2023
概括
在4°C储存时,聚合物阴离子体及其复合体显示出最佳的稳定性,用于基因传递. 储存在25°C或-20°C显著改变物理化学特性和转化效率.
科学领域:
- 生物技术是生物技术.
- 纳米技术 纳米技术
- 制药科学 制药科学
背景情况:
- 核酸复合物的稳定性对于基因传递应用至关重要,这种需求因疫苗储存挑战而放大.
- 作为基因载体的涅体的全面稳定性研究在科学文献中缺乏.
- 聚合物阴性阴性体正在成为有希望的非病毒基因传递载体.
研究的目的:
- 为了研究脂聚合物体和基于体的pCMS-EGFP复合物的稳定性.
- 评估不同储存温度 (4°C,25°C,-20°C) 对体物理化学性质和基因传递性能的影响.
- 确定基因基因传递系统的最佳存储条件.
主要方法:
- 尼奥索姆和尼奥普莱克斯在4°C,25°C和-20°C保存了8周.
- 评估了包括大小,泽塔潜力和多分散性指数 (PDI) 在内的物理化学性质.
- 在NT2细胞中评估了转染效率和细胞毒性.
主要成果:
- 与第0天相比,存储在25°C和-20°C的体显示出大小,泽塔潜力和PDI的显著变化.
- 在4°C下储存的体保持了合理的物理化学特性.
- 传染效率在4°C和-20°C保持稳定,但在25°C显著下降.
结论:
- 在4°C的存储条件下,可保持聚合物化和其复合物的物理化学完整性和转染效率.
- 体和体表现出在4°C下存储多达两个月的实际存储潜力,用于基因传递.
- 这项研究提供了这些阴茎细胞配方作为基因传递载体的稳定性和实用性的概念证明.
相关概念视频
00:46Genomic DNA in Prokaryotes
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
01:09Recombinant DNA
Overview
01:44Complementary DNA
Overview
02:57DNA-only Transposons
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
01:09Recombinant DNA
Overview
01:19Conjugation
Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...