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抑制BPHL可以抑制肺癌细胞系中的增殖

Pengfei Ren¹, Jianxue Zhai¹, Xuelian Wang²

¹Department of Thoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Translational lung cancer research

|June 16, 2023

概括

类似双氨酸酸酶 (BPHL) 通过增加扩散和转移来促进肺癌的生长,同时抑制亡. 击败BPHL显著减少瘤生长在体外和体内.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生物化学

背景情况:

肺癌是全球癌症死亡的主要原因之一.
类似双酸酶 (BPHL) 编码了一种参与前药物激活的酶.
BPHL在肺癌发病过程中的作用在很大程度上仍未被探索.

研究的目的:

研究BPHL在肺癌中的功能作用.
确定BPHL对癌细胞行为和瘤生长的影响.

主要方法:

利用短毛RNA (shRNA) 来抑制肺癌细胞系中的BPHL表达 (NCI-H1299和A549).
在试验室中评估了繁殖 (Celigo,MTT测定),亡 (Caspase 3/7活性),殖民地形成和转移 (Transwell测定).
分析了使用酸 (PI) 染色和光激活细胞分类 (FACS) 的细胞周期进展.
在体内使用异种移植小鼠模型评估瘤生长.

主要成果:

降低BPHL显著降低了肺癌细胞的增殖,殖民地形成和转移.
在BPHL中断后,亡显著增加.
在小鼠中,瘤生长在体内显著减少,这些小鼠具有BPHL-knockdown A549细胞.
细胞循环分析显示,在BPHL淘汰后,细胞循环分布发生了变化.

结论:

在肺癌中,BPHL起着促瘤的作用.
BPHL促进癌细胞的增殖,殖民地形成和转移.
BPHL抑制了细胞亡并促进了瘤生长,这表明它是潜在的治疗点.

关键词:

类似双酸酸酶的 (BPHL)抑制了扩散的发生.肺癌细胞系中的肺癌细胞系.

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