在瓜子中,Gibberellin生物合成是CPPU诱导的甲状腺炎所必需的
Yue Liu, Yang Li1, Huixin Guo1
1Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China.
Horticulture research
|June 16, 2023
概括
在瓜子 (Cucumis melo L.) 生产中应用细胞因素 (CK) CPPU,通过促进吉伯雷林 (GA) 生物合成,诱导果. 这项研究揭示了CPPU.
科学领域:
- 植物生物学 植物生物学
- 园艺园艺 园艺园艺
- 分子生物学分子生物学
背景情况:
- 细胞因素 (CK) CPPU通常用于西瓜果套.
- CPPU诱导的果的确切机制尚不清楚.
研究的目的:
- 为了阐明CPPU诱导的果 (Cucumis melo L.) 中的机制.
- 研究植物激素和基因表达在CPPU介导的水果发育中的作用.
主要方法:
- 瓜子水果的组织学和形态学观察.
- 激素分析测量吉伯雷林 (GA),奥克和酸 (ABA) 的水平.
- 转录组分析以确定差异表达的基因和途径.
- 应用GA抑制剂帕克洛布特拉 (PAC) 来评估GA的作用.
主要成果:
- 与正常授粉的水果相比,CPPU诱导的水果具有更高的细胞密度和更小的细胞大小.
- 在CPPU的应用增加了GA和auxin水平,同时在果期间降低了ABA.
- 抑制GA生物合成部分降低了CPPU诱导的果.
- 通过细胞因素信号通路组件CmRR2.2,CPPU通过基博瑞林生物合成基因CmGA20ox1进行上调.
结论:
- CPPU诱导的西瓜果严重依赖于吉伯雷林 (GA) 生物合成.
- 细胞因素信号通路,特别是CmRR2,积极调节GA生物合成基因表达.
- 这项研究为开发帕塞诺卡尔皮瓜品种提供了基本的理解.
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