iSort能够实现自动复杂的微流体滴滴分类,以实现技术民主化
Jatin Panwar1,2, Ramesh Utharala2, Laura Fennelly2
1Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Cell reports methods
|June 16, 2023
概括
本研究引入了使用实时阻抗分析的自动光激活滴滴分类 (FADS). 这项创新提高了单细胞分析的吞吐量和可重复性,使其更容易获得.
科学领域:
- 微流体学 微流体学
- 生物技术是生物技术.
- 一个单细胞分析.
背景情况:
- 光激活滴滴分类 (FADS) 对于高通量选至关重要,但需要专门的专业知识来优化参数.
- 目前的FADS方法在系统优化和跟踪单个滴滴方面面临挑战,导致分类准确性受到损害和隐藏的假阳性.
研究的目的:
- 开发一个用于实时优化FADS参数的自动化系统.
- 为了提高滴滴分类的吞吐量,可重复性和稳定性.
- 为了使表型单细胞分析更容易获得和更易于使用.
主要方法:
- 开发一个微流体设置,集成实时阻抗分析.
- 监测滴滴频率,间距和轨迹在分类结点.
- 基于阻抗数据实现自动化参数优化和干扰对抗.
主要成果:
- 实现了所有排序参数的连续,自动优化.
- 证明了更高的吞吐量,提高了可复制性,并在滴滴分类中提高了稳定性.
- 在实时中成功抵消干扰,提高排序可靠性.
结论:
- 开发的基于阻抗的自动化FADS系统克服了传统方法的主要局限性.
- 这一创新显著提高了表型单细胞分析的可访问性和性能.
- 该系统代表了单细胞分析平台广泛采用的重大进步.
相关概念视频
01:20Overview Of Cell Separation And Isolation
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
01:23Flow Cytometry
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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