相关实验视频

Updated: Jul 26, 2025

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
13:06

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

14.4K

快速,可扩展的组合基因组工程,通过无标记剂的丰富和酵母中的基因工程基因位点的再组合

Mudabir Abdullah1, Brittany M Greco1, Jon M Laurent2

  • 1Centre for Applied Synthetic Biology, Department of Biology, Concordia University, 7141 Sherbrooke St. W, Montreal, QC, Canada.

Cell reports methods
|June 16, 2023
PubMed
概括

我们开发了一种无标记基因组编辑方法,称为MERGE (无标记基因工程基因位点的无标记丰富和再组合) 用于酵母. 这种基于CRISPR-Cas9的方法有效地结合了多个基因编辑,使复杂的基因工程成为可能.

关键词:
克里斯普尔-Cas9是什么意思组合性基因组编辑是如何实现的基因驱动器的基因驱动器人性化的蛋白质酶体人性化的酵母酵母

更多相关视频

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

17.1K
Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

13.7K

相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K