在缺血性中风中对circRNA相关的ceRNA网络进行综合分析
Rongli Fan1, Die Hu1, Maiqiu Wang1
1Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products, Department of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, Zhejiang, China.
Frontiers in genetics
|June 16, 2023
概括
这项研究揭示了在缺血性中风 (IS) 中的一种新型循环RNA (circRNA) 网络. 这些发现突出了特定的circRNA-miRNA-mRNA轴,为IS诊断和预测提供了潜在的新生物标志物.
科学领域:
- 生物化学 生物化学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 缺血性中风 (IS) 是全球残疾和死亡的主要原因.
- 循环RNAs (circRNAs) 在IS病理生理学中起着重要作用,通常通过海绵式微RNAs (miRNAs) 来作为竞争的内源RNA (ceRNA) 起作用.
- 在IS中缺乏circRNA介导的ceRNA网络的全面分析.
研究的目的:
- 构建和分析与IS相关的整个转录组范围的circRNA-miRNA-mRNA ceRNA网络.
- 确定IS的关键监管轴和潜在生物标志物.
主要方法:
- 循环RNA,miRNA和信使RNA (mRNA) 的表达特征从基因表达综合 (GEO) 数据集中获得.
- 在IS患者中确定了差异表达 (DE) 分子.
- 像StarBase,CircBank和mirDIP这样的数据库被用于目标预测,以构建ceRNA网络.
- 蛋白质与蛋白质相互作用分析确定了枢纽基因和核心子网络.
主要成果:
- 分析发现了276个DEcircRNA,43个DEmiRNA和1926个DEmRNA.
- 构建了一个包含69个circRNA,24个miRNA和92个mRNA的ceRNA网络.
- 一个核心子网络包括关键分子,如hsa_circ_0011474,hsa_circ_0023110和基因,如CDKN1A,FHL2和RPS2.
结论:
- 一个新的ceRNA调节轴,hsa_circ_0011474 - hsa-miR-20a-5p/hsa-miR-17-5p - CDKN1A,与IS.有关,已经建立.
- 这些发现为IS的病原体提供了新的见解.
- 鉴定的circRNA和途径代表了IS的有前途的诊断和预测生物标志物.
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