相关实验视频

Updated: Jul 26, 2025

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

在抗EGFR治疗后对结直肠癌细胞表面标记物的SERS特征

Nana Lyu¹, Bernadette Pedersen², Elena Shklovskaya²

¹ARC Center of Excellence for Nanoscale BioPhotonics and School of Natural Sciences, Faculty of Science and Engineering Macquarie University Sydney New South Wales Australia.

Exploration (Beijing, China)

|June 16, 2023

概括

表面增强的拉曼光谱 (SERS) 有效地表征了结直肠癌 (CRC) 细胞. KRAS野生型CRC细胞对抗EGFR治疗的敏感性增加,HER2和HER3的表达减少.

科学领域:

在瘤学瘤学.
生物技术是生物技术.
频谱学是一种光谱学.

背景情况:

结肠直肠癌 (CRC) 是全球癌症死亡的主要原因.
在CRC中KRAS突变预测对抗EGFR疗法的反应不佳.
了解KRAS驱动的表型对于改善CRC治疗至关重要.

研究的目的:

在接受抗EGFR治疗的KRAS突变和野生型 (WT) CRC细胞的表型.
为了研究治疗期间细胞表面蛋白质表达的变化.
评估表面增强拉曼光谱法 (SERS) 作为生物标志物表征的方法.

主要方法:

使用抗体结合黄金纳米粒子 (纳米) 开发了一种SERS试验.
四种细胞表面蛋白质 (EpCAM,EGFR,HER2,HER3) 的表型被确定.
在24天内,KRAS突变 (SW480) 和WT (SW48) CRC细胞被用 cetuximab (抗EGFR) 治疗.

主要成果:

与KRAS突变细胞相比,KRAS WT细胞对抗EGFR治疗的敏感性更高.
在KRAS WT细胞中观察到HER2和HER3表达的显著下降.
通过流细胞计验证了SERS测定结果.

结论:

SERS是多重细胞表面生物标志物分析的一个有前途的方法.
在KRAS WT和突变CRC细胞的表型差异影响抗EGFR治疗反应.
通过单一的激光系统,SERS为细胞表面标记物的特征提供了一个潜在的替代方案.

关键词:

对抗EGFR的治疗方法结肠直肠癌是什么意思皮肤上生长因子受体.现型进化的演变.表面增强的拉曼光谱 (SERS)

更多相关视频

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Published on: January 22, 2021

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

相关概念视频

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...