猪的piRNA转录组对塞内卡病毒感染的反应是一种感染
Chen Wang1, Yanxi Chen1, Xiwang Yang1
1Southwest University, College of Veterinary Medicine, Chongqing, China.
Frontiers in veterinary science
|June 16, 2023
概括
塞内卡病毒A感染改变了猪细胞中的piRNA表达,可能影响昼夜节律和亡. 这项研究揭示了新的piRNA在宿主-病原体相互作用中的作用.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 塞内卡病毒A (SVA) 是一种皮科纳病毒,对宿主相互作用的了解很少.
- PIWI相互作用RNAs (piRNAs) 是小RNAs,在细胞过程中具有新兴的作用.
- 在SVA感染期间piRNAs的作用仍然在很大程度上是未知的.
研究的目的:
- 在SVA感染期间调查宿主细胞中的piRNA表达特征.
- 阐明SVA感染细胞中差异表达piRNAs的功能作用.
- 了解SVA对piRNA生物发生和相关途径的影响.
主要方法:
- 用RNA测序 (RNA-seq) 来识别SVA感染猪脏 (PK-15) 细胞中差异表达的piRNA.
- 使用定量逆转录PCR (qRT-PCR) 来验证关键piRNAs的表达.
- 进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
主要成果:
- 173个piRNA在感染SVA的PK-15细胞中被差异表达,10个通过qRT-PCR验证.
- 基因组分析表明,在新陈代谢,增殖和分化途径中含有丰富性.
- 凯格分析强调了AMPK,Rap1,昼夜节律和VEGF通路的丰富.
- 皮RNA生成基因BMAL1和CRY1的表达在感染后显著下调.
结论:
- 在PK-15细胞中,SVA感染显著改变了piRNA转录组.
- 差异表达的piRNAs参与调节抗病毒免疫力,平衡和潜在的瘤活动.
- 通过降低BMAL1和CRY1的调节,SVA可能会诱导亡并影响昼夜节律,这两种基因是产生piRNA的关键基因.
- 这项研究提供了第一份关于SVA感染期间PK-15细胞中的piRNA转录组的报告,进步了对piRNA调节机制的理解.
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