COVID-19 PCR:临床实践中内部控制抑制的频率
Alessandro C Pasqualotto1,2, Amanda L Seus3
1Federal University of Health Sciences of Porto Alegre (UFCSPA), Porto Alegre, Brazil.
Access microbiology
|June 16, 2023
概括
在COVID-19 PCR测试中,RNase P基因的内部控制显示了使用CDC协议的低抑制率 (2.4%). 这表明了协议的协议.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 传染性疾病 传染性疾病
背景情况:
- 实时定量PCR (qPCR) 是诊断COVID-19的黄金标准.
- 疾病预防控制中心的协议使用RNase P作为内部控制来验证SARS-CoV-2的qPCR结果.
- 内部控制对于检测抑制和确保测试准确性至关重要.
研究的目的:
- 在SARS-CoV-2 qPCR测试中确定RNase P基因抑制的频率.
- 评估CDC qPCR协议在巴西一家参考医院的有效性.
- 评估RNase P抑制对COVID-19诊断的影响.
主要方法:
- 通过qPCR对10311个临床样本进行了SARS-CoV-2检测的分析.
- 监测RNase P内部控制基因的放大.
- 根据平均周期值 (Ct) 和标准偏差计算抑制率.
主要成果:
- 总共有2.4%的样本表现出RNase P抑制.
- 在抑制样本中,69.4%的样本没有光,30.5%的样本有晚期放大.
- 对于RNase P的平均Ct值为26.65 (SD ±3.18). 对于RNase P的平均Ct值是26.65 (SD ±3.18).
结论:
- 美国疾病预防控制中心的qPCR协议在低RNase P抑制率下,证明了SARS-CoV-2检测的高有效性.
- 在COVID-19诊断PCR中,RNase P作为可靠的内部控制.
- 再提取是一种有效的策略,用于具有最小或没有RNase P光度的样品.
相关概念视频
01:13Controls in Experiments
7.9K
When conducting an experiment, it is crucial to have control to reduce bias and accurately measure the dependent variables. It also marks the results more reliable. Controls are elements in an experiment that have the same characteristics as the treatment groups but are not affected by the independent variable. By sorting these data into control and experimental conditions, the relationship between the dependent and independent variables can be drawn. A randomized experiment always includes a...
7.9K
02:57Real Time RT-PCR
57.5K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
57.5K