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在Corynebacterium glutamicum中设计和应用人造罕见的L-lysine编码子

Cuiping Yang¹, Zehao Peng¹, Lu Yang¹

¹Department of Biological Engineering, Qilu University of Technology, Jinan, China.

Frontiers in bioengineering and biotechnology

|June 16, 2023

概括

研究人员开发了一种新的方法,使用罕见的密码子来选高L-lysine产生的菌株. 这种技术在改造的Corynebacterium glutamicum中提高了L-lysine的产量高达9.7%.

科学领域:

微生物学微生物学
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

氨酸是一种重要的氨基酸,在料,食品和药品中广泛应用.
对于高L-lysine产生微生物菌株的有效选方法对于工业进步至关重要.

研究的目的:

开发一种高效准确的选方法,用于识别高L-lysine产生的菌株.
使用罕见的密码子技术,对Corynebacterium glutamicum进行工程,以提高L-lysine的生产.

主要方法:

在Corynebacterium glutamicum中通过tRNA促进体替代构建了罕见的L-lysine编码子AAA.
通过将增强绿色光蛋白 (EGFP) 密码子转化为AAA.开发了一个选标记物.
利用流细胞计和发酵来选具有增加L-lysine生产的高光突变物.

主要成果:

鉴定出55种突变体表现出更强的光,表明细胞内L-lysine含量更高.
与野生类型相比,在选定的突变菌株中,L-lysine产量的增加达到了9.7%.
显示了69%的最高查阳性率.

结论:

人工构建的罕见密码子提供了一个高效,准确和简单的选工具.
该方法适用于选其他生产氨基酸的微生物.
开发的技术有助于实现识别优质微生物菌株的工业目标.

关键词:

在Corynebacterium glutamicum的研究中,我们发现对于L-lysine来说,这是一个非常重要的药物.基因淘汰赛基因淘汰赛高流量的高流量.非常罕见的鱼.

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