爆米花 (Zea mays L) 不同. ) 由纳瓦霍族表型和 ploidy 级别识别的单体
Jean Paulo Aparecido da Silva1, José Marcelo Soriano Viana1, Kaio Olimpio das Graças Dias1
1Department of General Biology, Federal University of Viçosa, Viçosa, Brazil.
Frontiers in plant science
|June 16, 2023
概括
这项研究开发了使用克拉斯诺达尔哈普洛伊德诱导器 (KHI) 诱导和识别爆米花中的哈普洛伊德的方法. 结果显示平分体诱导率较低,但强调了纳瓦霍族表型和流细胞测量的有效性,用于准确的平分体识别.
科学领域:
- 植物育种和遗传学
- 农作物科学 农作物科学
- 分子生物学分子生物学
背景情况:
- 哈普洛伊德诱导和识别是爆米花育种中的关键但具有挑战性的步骤.
- 开发用于爆米花混合繁殖的双倍哈普洛伊德技术需要克服与玉米诱导物的交叉不兼容性.
研究的目的:
- 用纳瓦霍族的表型,幼苗的活力和 ploidy 水平来诱导和选爆米花中的 haploids.
- 通过使用克拉斯诺达尔哈普洛伊德诱导器 (KHI) 评估哈普洛伊德诱导和识别的有效性.
- 评估纳瓦霍标记基因 (R1-nj) 的透性及其在平分体查中的实用性.
主要方法:
- 使用KHI对20个爆米花胚胎和5个玉米对照进行了交叉,在随机的完整块设计中进行了交叉.
- 评估了状体诱导率 (HIR),虚假阳性率 (FPR) 和虚假阴性率 (FNR).
- 使用纳瓦霍表型 (R1-nj),苗木活力,并通过流细胞计证实 ploidy 水平.
主要成果:
- 该KHI实现了0.34%的平均HIR,范围为0.0-1.2%.
- 纳瓦霍族的表型查显示,平均FPR为26.2%的活力和76.4%的力;FNR为0%.
- R1-nj的透率从30.8%到98.6%不等,受生殖质起源和遗传背景的影响.
结论:
- 在热带和温带地区起源的爆米花生殖质中,哈普洛伊德诱导是可行的.
- 建议将纳瓦霍表型与流细胞计结合起来,以获得准确的单 haploid 选择.
- 基于纳瓦霍表型和幼苗活力的选方法有效地减少了错误分类,但克服交叉不兼容性是爆米花双倍平分类技术的关键.
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