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茄中Trichoderma citrinoviride病毒性和减弱菌株介导了耐药性和生物控制机制

Di Zhao¹, Dan Shen², Haiyan Fan²

¹Analysis and Testing Center, Shenyang Agricultural University, Shenyang, China.

Frontiers in plant science

|June 16, 2023

概括

在番茄中,Trichoderma citrinoviride菌株提供了对根结线虫的双重控制. 毒性菌株直接杀死线虫,而减弱菌株诱导植物的抗性,揭示了复杂的生物控制机制.

科学领域:

植物病理学植物病理学
微生物生物控制微生物生物控制
遗体学遗体学是一个学科.

背景情况:

根结线虫病对全球蔬菜生产构成重大威胁.
三体物种越来越多地被用作对抗植物寄生虫线虫的生物控制剂.

研究的目的:

确定番茄植物中毒和减弱的Trichoderma citrinoviride菌株介导的耐药性和生物控制机制.
阐明Trichoderma citrinoviride菌株对Meloidogyne incognita和番茄防御通路的差异性影响.

主要方法:

用毒性 (T1910) 和减弱的 (TC9) 皮虫 (Trichoderma citrinoviride) 菌株对抗Meloidogyne incognita juveniles (J2s) 进行了杀虫毒性测定.
盆栽实验评估了这些菌株在控制番茄植物中M.隐形菌的有效性.
使用定量实时PCR (qRT-PCR) 分析番茄防御相关基因的表达.

主要成果:

毒性菌株T1910与减弱菌株TC9 (23.01%的死亡率,LC50=2.0615) 相比,表现出明显更高的杀菌活性 (92.37%的死亡率,LC50=0.5585).
实验显示,毒菌株 (85.22%,76.91%) 与减弱菌株 (63.16%,59.17%) 相比,在番茄根胆中,M. incognita J2和J4阶段的抑制率更高.
基因表达分析揭示了不同的防御通路激活:TC9提升了更早 (5天后注射) 的防御基因 (LOX1,PR1,PDF1.2),而T1910诱导了更强的PR5提升和稍后,较弱的JA通路激活.

结论:

特里科德玛-西特里诺维里德采用双重生物控制策略:通过毒性菌株直接毒性,并通过减弱菌株诱导耐药性.
减弱菌株TC9通过线虫相关的分子模式触发的免疫引发了较早的番茄免疫反应.
在Trichoderma中的毒性降解并没有消除其诱导植物耐药性的能力,突出了多功能生物控制潜力.

关键词:

三皮虫 (Trichoderma citrinoviride) 是一种有毒的植物.减弱菌株是一种减弱菌株.生物控制机制生物控制机制介导电阻是介导电阻的介导电阻.毒性菌株是一种毒性菌株.

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