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黑色素调节下丘脑-垂体神经内分泌轴,以调节远鱼的生理色彩变化

Jiaqian Feng¹, Jingwen Yang¹, Zhijing Jiang¹

¹National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science College, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, People's Republic of China.

International journal of biological sciences

|June 16, 2023

概括

鱼类中的黑色素信号传递涉及五个受体,激活ERK1/2通路. 这项研究揭示了通过下丘脑-垂体轴调节鱼类颜色变化的新途径.

科学领域:

内分泌学在内分泌学.
神经科学是一个神经科学.
海洋生物学海洋生物学

背景情况:

黑素 (MT) 调节脊椎动物生理学,包括昼夜节律.
远距离的MT信号系统在很大程度上没有特征.
大型黄色的croaker表现为昼夜的身体颜色变化.

研究的目的:

为了功能性地研究远距离的MT信号系统在大黄色的croaker.
为了阐明MT受体的细胞内信号通路.
揭示MT信号在下丘脑-垂体神经内分泌轴和颜色变化中的作用.

主要方法:

通过MT对五个已识别的MT受体 (LcMtnr1a1,LcMtnr1a2,LcMtnr1b1,LcMtnr1b2,LcMtnr1c) 的激活试验进行了测试.
分析ERK1/2酸化和G蛋白合途径 (Gαi,Gαq,Gαs).
使用单细胞RNA-seq和空间表达数据构建信号模型;通过药理实验验验证.

主要成果:

所有五个MT受体都通过不同的G蛋白通路激活了ERK1/2酸化.
LcMtnr1a2和LcMtnr1c是Gαi依赖的; LcMtnr1b对应物是Gαq依赖的; LcMtnr1a1是Gαi/Gαs依赖的.
确定了一种新型的MT/MCH和MT/TAC1/CRH/MSH通路,调节染色体调动和颜色变化.

结论:

多个细胞内信号通路由L. crocea的黑素受体进行介导.
MT信号系统在海洋teleosts的垂体下垂体神经内分泌轴上游发挥作用.
这项研究为MT在鱼类染色体调动和生理色彩变化中的作用提供了关键的见解.

关键词:

细胞信号传递是细胞的信号传递.染色体是染色体的组成部分.下垂体 - 下垂体轴.黑色素受体的受体是什么一个单细胞RNA-seqq.

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