矩阵生成细胞诱导了Bacillus subtilis生物膜中的形态差异
Xianyong Li1, Rui Kong1, Jiankun Wang1
1School of Mechanical Engineering, University of Science and Technology Beijing, Beijing, 100083 China.
Indian journal of microbiology
|June 16, 2023
概括
细菌细菌生物膜表现出一个独特的"咖啡环"结构. 细胞外矩阵分布和细胞表型解释了这种环形形成和由此产生的生物膜内的形态变异.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
背景情况:
- 细菌细菌生物膜表现出独特的"咖啡环"形态.
- 在这个"咖啡环"内外观察到不同的殖民地生物膜结构.
研究的目的:
- 研究Bacillus subtilis生物膜中"咖啡环"形成的原因.
- 解释与"咖啡环"结构相关的形态变异.
主要方法:
- 开发了一种定量方法来表征生物膜表面形态.
- 采用物流增长模型来评估环境阻力对生物膜厚度的影响.
- 利用光学成像和BRISK算法来追踪运动细胞和产生基质的细胞.
主要成果:
- "咖啡环"的外部区域比内部区域更厚,厚度幅度更大.
- 死亡的细胞会产生释放压力的隙,导致生物膜中的折叠形成.
- 产生基质的细胞集中在"咖啡环"外,细胞外基质 (ECM) 阻碍细胞向外移动.
- 运动细胞主要位于环内,而外部的死亡运动细胞有助于放射性折叠的形成.
- 环内部没有ECM,可以形成均的折叠.
结论:
- 细胞外矩阵的空间分布和不同的细胞表型是Bacillus subtilis中"咖啡环"形成的关键驱动因素.
- 使用eps和鞭毛突变体的实验验证支持了拟议的机制.
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