相关实验视频

Updated: Jul 26, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

与PIWI相互作用的RNA-17458具有致癌性,是宫癌的潜在治疗标

Lianqin Liu¹, Liu Li², Wufan Zu³

¹Department of Pathology, Henan Provincial People's Hospital; People's Hospital of Zhengzhou University; People's Hospital of Henan University, Zhengzhou, Henan, 450003, the People's Republic of China.

Journal of Cancer

|June 16, 2023

概括

皮维相互作用RNA (piRNA) -17458在子宫癌 (CC) 中过度表达. 这种piRNA通过调节WTAP介导的N6-甲基氨酸 (m6A) 甲基化来促进CC细胞的增殖,迁移和入侵.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

子宫癌 (CC) 是一个重要的生殖系统癌症.
在CC病变发生过程中,piwi相互作用RNA (piRNA) 的作用尚不清楚.
了解piRNA机制对于开发新型CC疗法至关重要.

研究的目的:

研究piRNA-17458在宫癌中的作用和机制.
为了确定piRNA-17458,WTAP和CC中的m6A修饰之间的关系.

主要方法:

定量实时PCR评估piRNA-17458在CC组织和细胞中的表达.
在体外测试 (模仿/抑制剂) 以评估piRNA-17458对CC细胞增殖,迁移和入侵的影响.
在体内外移植的老鼠模型来评估瘤生长.
西方涂抹和双露西法酶记者试验,以调查piRNA-17458和WTAP之间的相互作用.
N6-甲基氨酸 (m6A) RNA免疫沉试验测量m6A水平.

主要成果:

在CC组织和细胞中,piRNA-17458显著过度表达.
piRNA-17458在体内促进了CC细胞的增殖,迁移和入侵以及瘤的生长.
piRNA-17458增强了CC细胞中的m6A水平和WTAP稳定性.
确定WTAP是piRNA-17458.8的一个直接目标.
淘汰WTAP扭转了piRNA-17458.8的前瘤性影响.

结论:

在宫癌中,piRNA-17458是致癌的.
piRNA-17458通过一种涉及WTAP介导m6A甲基化的机制促进CC瘤发生.
这些发现突出了piRNA-17458作为宫癌的潜在治疗点.

关键词:

在WTAP中,使用的是WTAP.宫癌:子宫癌是一种癌症.m6ARNA甲基化的过程恶性瘤是一种恶性瘤.这就是piRNA-174588.

更多相关视频

Bacterial Delivery of RNAi Effectors: Transkingdom RNAi

Bacterial Delivery of RNAi Effectors: Transkingdom RNAi

Published on: August 18, 2010

DNA Vector-based RNA Interference to Study Gene Function in Cancer

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

相关概念视频

piRNA - Piwi-interacting RNAs

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Non-LTR Retrotransposons

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...