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电子舌头用于确定人类病原细菌检测极限

Aya Abu Rumaila¹, Basima Abu Rumaila¹, Wafa Masoud¹

¹Department of Agricultural Biotechnology, Faculty of Agricultural Sciences and Technology, Palestine Technical University-Kadoorie (PTUK), P.O. Box 7, Jaffa Street, Tulkarm, Palestine.

|June 16, 2023

概括

电子舌头 (ET) 可以检测食物传播的细菌,如金黄色葡萄球菌和大肠杆菌在非常低的度. 这种强大的诊断工具可以早期识别病原体,有助于及时治疗患者.

科学领域:

生物技术和生物传感器技术
食品安全与微生物学
分析化学和传感器阵列

背景情况:

电子舌头 (ET) 是一种使用具有高交叉灵敏度的多传感器阵列的诊断工具.
目前用于食品传播病原体的诊断方法可能耗时且需要样本准备.
早期检测细菌增殖和代谢变化对于及时干预至关重要.

研究的目的:

研究使用Astree II Alpha MOS电子舌头用于早期检测食物传播细菌.
为了确定Staphylococcus aureus和大肠杆菌的检测极限 (LOD).
用预先存储的模型评估ET识别未知细菌样本的能力.

主要方法:

细菌培养S. aureus和大肠杆菌被连续稀释.
用Astree II Alpha MOS电子舌头分析了稀释的样本.
部分最小方形 (PLS) 回归和主要组件分析 (PCA) 用于数据分析.

主要成果:

电子舌头检测到细菌的增殖和代谢变化在1011到1010的稀释.
在化6小时后检测到金黄色细菌,在6到8小时之间检测到大肠杆菌.
ET成功地根据它们独特的代谢概况对未知的细菌样本进行了分类.

结论:

阿斯特里II电子舌头是一种强大的潜度测量工具,用于早期识别食源微生物.
ET可以在复杂系统中的原生状态中进行检测,从而促进快速诊断.
这项技术具有改善食品安全和患者治疗结果的巨大潜力.

关键词:

电子舌头是一种电子舌头通过食物传播的病原体.多变量数据分析.部分最小正方形.主要组件分析的主要组件分析

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