一种快速演变的单复制基因组H1变体与寄生虫黄蜂的雄性生育有关
Bo Yuan1, Yi Yang1, Zhichao Yan1
1State Key Laboratory of Rice Biology and Breeding and Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Frontiers in cell and developmental biology
|June 16, 2023
概括
在寄生虫黄蜂丸中发现了两种新的组素H1变体 (PpH1V1和PpH1V2). PpH1V1的干扰影响了精子的生育能力,揭示了昆虫体育生殖中的不同角色.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 进化生物学 进化生物学
背景情况:
- 基因组H1变异对染色质结构和专门的细胞功能至关重要.
- 特定于生殖系的H1变异在物种间的游戏生成中发挥着不同的作用.
- 关于昆虫生殖系特定的H1变体的知识是有限的,特别是在非模型生物中.
研究的目的:
- 在寄生虫黄蜂*Pteromalus puparum*中识别和表征生殖系特定的H1变异.
- 为了研究这些H1变异的进化动态在Hymenoptera.
- 阐明 *PpH1V1* 和 *PpH1V2* 在精子生成和男性生育能力中的功能作用.
主要方法:
- 通过基因表达分析主要在丸中识别H1变异.
- 进化分析以确定海门的副本数量和进化速率.
- 通过RNA干扰 (RNAi) 实验来破坏*PpH1V1*和*PpH1V2*的功能.
- 精子生成,染色质结构和精子生育能力的表型分析.
主要成果:
- 两种H1变体, *PpH1V1* 和 *PpH1V2*,被确定为主要表达在 *P. puparum* 丸中.
- 进化分析表明,这些H1变异基因的快速进化和单一副本维护在白鸟中.
- *PpH1V1* 干扰导致异常的染色质结构和精子生育能力降低,尽管精子生成正常.
- *PpH1V2* knockdown 对男性生育能力或精子生成没有显著影响.
结论:
- 这项研究在非模型昆虫Pteromalus puparum*中发现了新的生殖系特异性的H1变异.
- 在 *Pteromalus* 和 *Drosophila* 之间观察到男性生殖系丰富的H1变体的独特功能.
- 这些发现突显了动物生殖系特异性H1蛋白的功能复杂性和进化多样性.
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