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CD39取消了血小板衍生因素诱导的人类胎盘IL-1β表达

Désirée Forstner¹, Jacqueline Guettler¹, Beatrice A Brugger¹

¹Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria.

Frontiers in cell and developmental biology

|June 16, 2023

概括

怀孕期间胎盘CD39表达的增加,特别是在孕前,有助于分解细胞外ATP. 这个过程可以作为一种抗凝固防御,减少炎症和血小板激活.

科学领域:

生殖生物学生殖生物学
免疫学免疫学免疫学
生物化学生物化学

背景情况:

在组织攻击期间释放的细胞外ATP调节炎症和血小板激活.
在怀孕期间增强ATP水解,这表明它具有抗炎作用.
CD39和CD73酶将细胞外ATP转化为AMP,然后转化为腺.

研究的目的:

在怀孕期间调查胎盘CD39和CD73表达的发育变化.
为了比较产前的表达与健康怀孕的表达.
分析由血小板因子和胎盘扩张体和BeWo细胞中的氧气水平的调节.

主要方法:

对胎盘CD39和CD73表达的线性回归分析.
免疫组织化学定位CD39和CD73.
在不同的氧气和血小板释放条件下培养胎盘扩展体和BeWo细胞.
在BeWo细胞中过度表达复合人类CD39.

主要成果:

胎盘CD39增加,而CD73在期末前下降.
CD39和CD73被局部定位到同胞性细胞中.
孕前症显著增加了胎盘CD39和CD73的表达.
血小板释放,但没有氧气水平,放松了CD39的表达.
在暴露于血小板因子的BeWo细胞中,CD39过度表达减少了细胞外ATP和IL-1β.

结论:

在孕前症中,胎盘CD39升调表明需要增加ATP水解.
CD39对血小板因子的反应可能会增强ATP转化,作为抗凝固剂的胎盘防御.

关键词:

腺三酸盐 (ATP) 是一种这些是ectonucleotidases.胎盘胎盘胎盘胎盘血小板衍生因素血小板衍生因素预埃克兰普西亚是什么意思

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