在Staphylococcus aureus感染期间,非免疫细胞对先天免疫记忆的发展取决于活性氧物种
Emmanuel Chaumond1, Sandrine Peron1, Nathalie Daniel1
1l'Institut national de recherche pour l'agriculture, l'alimentation et l'environnement (INRAE), Institut Agro, Science et Technologie du Lait et de l'Oeuf (STLO), Rennes, France.
Frontiers in immunology
|June 16, 2023
概括
天生的免疫记忆可以诱导非免疫细胞对抗黄金葡萄球菌感染. 像Lactococcus lactis这样的益生菌显示出开发针对S. aureus的新疗法的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 微生物学 微生物学
背景情况:
- 天生的免疫记忆,或训练免疫,涉及免疫细胞的表观遗传和代谢变化.
- 在非免疫细胞中训练免疫的机制仍然不太清楚.
- 黄金葡萄球菌会导致难以治疗的感染,这凸显了对新型治疗策略的需求.
研究的目的:
- 为了研究在黄金葡萄球菌感染期间非免疫细胞中先天免疫记忆的发展.
- 探索非免疫细胞中先天免疫记忆的潜在诱导因素.
主要方法:
- 使用了酶相关免疫吸收试验 (ELISA),显微镜分析和细胞计量.
- 训练有素的人类骨质细胞样MG-63和肺上皮细胞A549细胞与β-葡萄糖和乳球菌乳酸菌.
- 评估了细胞因子的产生 (IL-6,IL-8) 和组织蛋白修饰 (H3K27乙化).
主要成果:
- 贝塔葡萄糖训练增强了IL-6和IL-8的产生,以应对S. aureus,与H3K27乙化有关.
- 反应性氧物种 (ROS) 参与天生的免疫记忆诱导.
- 乳球菌还诱导非免疫细胞的先天免疫记忆,与H3K27乙化相关.
结论:
- 这项研究证明了非免疫细胞对黄金色杆菌的先天免疫记忆.
- 益生菌,如乳糖菌,是潜在的诱导先天免疫记忆.
- 结果可能会为S. aureus感染的替代治疗策略提供信息.
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