ZmEREB57调节OPDA合成,并通过Zea mays的两个不同的信号通路提高盐应激耐受性
Jiantang Zhu1, Xuening Wei1, Chaoshu Yin1
1School of Biological Science and Technology, University of Jinan, Jinan, China.
Plant, cell & environment
|June 16, 2023
概括
玉米APETALA2/乙烯反应因子 (AP2/ERF) 转录因子ZmEREB57提高了盐分耐受性. 它调节了12-oxo-phytodienoic acid (OPDA) 和jasmonic acid (JA) 信号通路,这对于植物应激反应至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 压力生理学 压力生理学
背景情况:
- APETALA2/乙烯反应因子 (AP2/ERF) 转录因子是植物非生物应激耐受性的关键调节者.
- 了解这些因素在玉米等作物中的具体作用,对于提高性至关重要.
研究的目的:
- 鉴定和描述玉米AP2/ERF基因ZmEREB57在应对非生物应激,特别是盐应激时的功能.
- 为了阐明ZmEREB57介导的盐耐受性背后的分子机制.
主要方法:
- 在玉米和Arabidopsis中克隆和描述ZmEREB57的基因.
- CRISPR/Cas9基因编辑以创建淘汰线和过度表达研究.
- 用DNA亲和力净化测序 (DAP-Seq) 来识别ZmEREB57的目标基因.
- 对贾斯蒙酸 (JA) 和12-oxo-phytodienoic acid (OPDA) 途径进行转录组分析和突变分析.
主要成果:
- ZmEREB57是一种核蛋白,具有转换活化活性,由非生物压力诱导.
- ZmEREB57淘汰赛线表现出增加的盐敏感性,而过度表达增强了盐耐受性.
- ZmEREB57与ZmAOC2的促进体结合,调节OPDA和JA的合成.
- 在盐反应中,OPDA 起到信号分子的作用,其功能独立于 JA.
结论:
- 在玉米中,ZmEREB57在赋予盐耐受性方面发挥着重要作用.
- 该机制涉及OPDA和JA信号通路的调节.
- OPDA信号是植物盐应激反应的关键组成部分.
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