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按菌中的尼古丁Agaricus bisporus,内源生物合成?
Daan Van Elst1, Els Van Pamel1, Patrick Sedeyn2
1Flanders Research Institute for Agriculture, Fisheries and Food, Technology and Food Science Unit, Melle, Belgium.
概括
这项研究调查了像Agaricus bisporus这样的是否自然产生尼古丁. 结果显示,不会合成尼古丁,这表明外部污染是可能的来源.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物生物化学 植物生物化学
- 食品科学 食品科学 食品科学
背景情况:
- 2009年,尼古丁在干燥的样本中出乎意料地被检测到.
- 中尼古丁的来源尚不清楚,这促使人们对内源合成进行调查.
研究的目的:
- 为了确定*Agaricus bisporus* (常见的) 是否可以内源性合成尼古丁.
- 在各种条件下分析中的尼古丁及其前体 (素,尼古丁酸).
主要方法:
- 在受控,无尼古丁的环境中培养*Agaricus bisporus*.
- 分析新鲜,储存,完整和加工的,使用超高性能液体染色学-并联质谱法 (UHPLC-MS/MS).
- 在A. bisporus*进行*in silico*基因组分析.
主要成果:
- 没有发现内源性尼古丁生物合成的证据,即使在储存或加工后 (检测极限为1.6 ng g-1).
- 所有样本中都存在普特雷辛和尼古丁酸,不同治疗方法的含量有所不同.
- 基因组分析证实 *A. bisporus* 缺乏生产尼古丁的遗传机制.
结论:
- 这项研究没有提供中天然内源尼古丁的证据.
- 样本中尼古丁的存在很可能是由于外源污染.
- 潜在的污染源包括手工挑选,样品准备或分析程序.
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