在SARS-CoV-2感染的临床过程和ACE2和TMPRSS2表达和多形态之间的关系
Esra Tug1, Isil Fidan2, Gulendam Bozdayi2
1Department of Medical Genetics, Medical University of Gazi, Turkey.
概括
主体ACE2和TMPRSS2的遗传变异影响COVID-19的易感性和严重程度. 特定的基因表达和多态性与SARS-CoV-2感染相关,影响疾病的过程并提供潜在的治疗点.
科学领域:
- 遗传学 是一个遗传学.
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 主体遗传因素,包括病毒尖峰 (S) 蛋白,ACE2和TMPRSS2的变异,可能会影响SARS-CoV-2感染的易感性和疾病严重程度.
- 了解这些遗传决定因素对于理解COVID-19病原体至关重要.
研究的目的:
- 调查ACE2和TMPRSS2基因表达模式和多态性与COVID-19临床结果之间的关联.
- 为了确定SARS-CoV-2易感性和疾病进展的潜在遗传标记.
主要方法:
- 检查了147名COVID-19患者 (无症状,有症状,ICU) 和33名健康对照.
- 使用RT-qPCR评估了ACE2和TMPRSS2基因表达.
- 通过RT-qPCR分析了ACE2和TMPRSS2的基因型分布和单核酸多态 (SNP).
主要成果:
- 在SARS-CoV-2-阳性和阴性组之间观察到ACE2和TMPRSS2表达的显著差异.
- 特定的ACE2基因型 (rs714205GG) 和TMPRSS2基因型/亚型型 (rs8134378GA,rs2070788GA,rs7364083GA,rs9974589AC,CTTA,AGCAG,AGAAG) 与SARS-CoV-2阳性和临床表现相关.
- 在症状患者中,等位基表达 (rs1978124 C-等位基,rs8134378 A-等位基) 是显著的.
结论:
- 宿主ACE2和TMPRSS2的遗传变异与COVID-19易感性和临床过程有关.
- 识别这些遗传关联可以为新型疫苗和治疗策略的开发提供信息.
- 对宿主-病原体遗传相互作用的进一步研究是有必要的,以改善COVID-19管理.
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