一种基基基酸甲前化酶表现出对前捐赠者的乱交性
Andreas Martin1, Nele Dierlamm2, Georg Zocher2
1Institut für Pharmazeutische Biologie und Biotechnologie, Fachbereich Pharmazie, Philipps-Universität Marburg, Robert-Koch Straße 4, 35037, Marburg, Germany.
Applied microbiology and biotechnology
|June 16, 2023
概括
这项研究表明,真菌前转移酶ShPT接受氨酸乙烯的前化,而不是醇. 这扩大了用于创建前化乙烯衍生物的工具.
科学领域:
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
- 有机化学 有机化学
背景情况:
- 甲基转移酶是催化甲基转移的酶,在天然产品生物合成中至关重要.
- 以前认为,来自Stereum hirsutum的真菌前转移酶ShPT参与了通过前化4-基醇参与振动素生物合成.
- 菌群之一的基菌群 (Basidiomycetes) 是一个较少探索的前转移酶的来源.
研究的目的:
- 为了生物化学地表征真菌前转移酶ShPT.
- 为了确定ShPT的基质特异性.
- 扩大化学工具箱,用于合成前化乙烯衍生物.
主要方法:
- 使用ShPT对各种基质进行酶检测,包括氨酸,醇和甲基.
- 使用了二甲基亚利二酸盐和日拉尼尔二酸盐作为先供体.
- 描述了前化反应的区域选择性.
主要成果:
- 与之前的假设相反,ShPT接受氨衍生物用于C-prenylation.
- 在测试条件下,醇和化物没有被ShPT先化.
- 该酶证明了氧纳乙烯的区域选择性前化.
结论:
- ShPT是Stereum hirsutum的一种先转移酶,首选的是先酸酸,而不是醇.
- 虽然ShPT的天然基质仍然未被确定,但它的活性扩大了已知从基菌体中获得的prenyltransferase的范围.
- 这项研究提供了一种有价值的方法,用于预化甲烯化合物的区域选择性合成.
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