一种新型细菌菌体vB_Pci_PCMW57的完整基因组序列感染了植物细菌 pseudomonas cichorii
Márcia Keller Alves1, Wellington Vieira de Souza2, Júnia Capua de Lima Novello3
1Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil. marcia_nutri@hotmail.com.
Molecular biology reports
|June 16, 2023
概括
一种新型的菌体,vB_Pci_PCMW57,被发现感染了Pseudomonas cichorii,一种导致重大作物疾病的植物病原体. 这标志着首次报告P. cichorii特有的菌体,提供了潜在的生物控制策略.
科学领域:
- 微生物学 微生物学
- 植物病理学 植物病理学
- 病毒学 病毒学
背景情况:
- 伪菌 (Pseudomonas cichorii,P. cichorii) 是一种格拉姆阴性植物细菌,在全球引起重大植物疾病.
- 这种细菌经常出现在叶菜中,对农业构成威胁.
研究的目的:
- 孤立和描述一种针对植物病原体Pseudomonas cichorii的新型细菌菌体.
- 提供第一个能够感染P. cichorii. 的菌体的基因组和形态描述.
主要方法:
- 从叶菜样本中分离细菌菌体.
- 使用电子显微镜对病毒体结构进行表征.
- 全基因组测序以确定基因组大小,GC含量,并打开阅读框架.
主要成果:
- 一种新的毒性细菌菌体,vB_Pci_PCMW57,被分离出来,是P. cichorii的特异性.
- 电子显微镜揭示了一个小的病毒体,具有一个icosahedral体和一个短的尾巴.
- 菌体基因组为40,117bp,具有49个开放的读取,显示高序列相同性 (>95%) 与其他Pseudomonas菌体.
结论:
- vB_Pci_PCMW57被归类为Caudoviticetes,Autographiviridae家族,Studiervirinae亚家族中的一个.
- 这项研究报告了第一个感染Pseudomonas cichorii的细菌被分离出来.
- 这些发现有助于了解菌体的多样性和在控制P. cichorii植物疾病方面的潜在应用.
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