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在肺腺癌中识别新的预后性长非编码RNA,使用WGCNA分析

Can Zhao¹, Han Xu¹, Chang Liu²

¹Department of Thoracic Surgery, Liaoning Health Industry Group Fukuang General Hospital, Liaoning, 113001, China.

Biochemical genetics

|June 16, 2023

概括

长非编码RNA对肺腺癌 (LUAD) 的发展至关重要. 这项研究确定了三个关键的lncRNAs (MIR99AHG,ADAMTS9-AS2,AC037459.2) 和19个mRNAs作为LUAD患者有前途的预后生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

长非编码RNAs (lncRNAs) 越来越多地被认为是它们在肺腺癌 (LUAD) 瘤发生中的作用.
在LUAD中的许多lncRNAs的特定功能在很大程度上仍然没有特征,突出了我们对LUAD进展的理解上的差距.

研究的目的:

确定新的长非编码RNAs (lncRNAs) 和信使RNAs (mRNAs) 作为肺腺癌 (LUAD) 的预后生物标志物.
使用生物信息学方法探索关键基因模块在LUAD预后中的功能作用.

主要方法:

权重基因相关性网络分析 (WGCNA) 用于在TCGA-LUAD队列中构建基因共同表达模块.
蛋白质与蛋白质相互作用 (PPI) 网络,基因本体学 (GO) 和KEGG通路分析被用于调查已识别的关键模块的功能.
建立了一个mRNA-lncRNA联合表达网络,以精确确定与LUAD预后相关的关键 lncRNA.

主要成果:

一个包含130个基因的关键模块 (Tan模块) 被确定与LUAD预后有显著的相关性.
关键模块中的基因被发现在十个不同的信号通路中被丰富.
三种特定的lncRNAs (MIR99AHG,ADAMTS9-AS2,AC037459.2) 和19种mRNAs被确定为LUAD.的潜在预后生物标志物.

结论:

已识别的lncRNA和mRNA为LUAD的预后监测提供了新的见解.
这些生物标志物有潜力开发向治疗和改善肺腺癌的临床管理策略.

关键词:

长的非编码RNA是什么?肺部腺癌是肺部的癌症.预测生物标志物预测生物标志物在WGCNA中,WGCNA是WGCNA.在cnRNA-mRNA网络中.

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