Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Biodegradability of Acrylate-Lipoic Acid Copolymers.

Journal of the American Chemical Society·2026

Same author

Engineered Gram-Positive Based Quorum Sensing for Metabolic Control in <i>Escherichia coli</i>.

ACS synthetic biology·2025

Same author

Deciphering allosterism of an <i>Escherichia coli</i> hexuronate metabolism regulator: UxuR.

RSC medicinal chemistry·2025

Same author

Optimization of the Isopentenol Utilization Pathway for Isoprenoid Synthesis in <i>Escherichia coli</i>.

Journal of agricultural and food chemistry·2022

Same author

Implementation of Synthetic Pathways to Foster Microbe-Based Production of Non-Naturally Occurring Carboxylic Acids and Derivatives.

Journal of fungi (Basel, Switzerland)·2021

Same author

Prospecting Biochemical Pathways to Implement Microbe-Based Production of the New-to-Nature Platform Chemical Levulinic Acid.

ACS synthetic biology·2021

Search research articles

相关实验视频

Updated: Jul 4, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

生物传感器开发用于单细胞检测葡萄糖酸盐.

Jennifer Kaczmarek Nash¹, Kristala L J Prather¹

¹Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139,USA.

Journal of industrial microbiology & biotechnology

|June 16, 2023

概括

此摘要是机器生成的。

这项研究优化了一种转录因子生物传感器,用于选myo-inositol oxygenase (MIOX) 变种. 修改改进了生物传感器性能,使密切相关的MIOX同类物能够分离.

关键词:

生物传感器是一种生物传感器.定向进化是指导进化的.代谢工程是代谢工程.代谢物敏感转录因子的转录因子合成生物学合成生物学

更多相关视频

FIBS-enabled Noninvasive Metabolic Profiling

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Published on: April 13, 2019

相关实验视频

Last Updated: Jul 4, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

FIBS-enabled Noninvasive Metabolic Profiling

FIBS-enabled Noninvasive Metabolic Profiling

Published on: February 3, 2014

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Published on: April 13, 2019

科学领域:

生物技术是生物技术.
分子生物学分子生物学
酶工程是什么? 酶工程是什么?

背景情况:

生物传感器为遗传图书馆提供了高通量选能力.
生理学限制和机械学知识缺口可能会阻碍生物传感器应用,类似于微生物系统.
基于转录因子的生物传感器,就像ExuR系统一样,是合成生物学中宝贵的工具.

研究的目的:

为了对先前开发的基于转录因子 (ExuR) 的银河酸盐生物传感器及其相关联体葡萄糖的特征.
用不同的myo-inositol氧化酶 (MIOX) 同类物来研究生物传感器的性能.
优化生物传感器电路架构和培养条件,以提高MIOX变种查的性能和应用.

主要方法:

基于ExuR的葡萄糖生物传感器的表征.
测试各种MIOX同类生物传感器响应.
修改生物传感器电路架构和培养条件.
优化生物传感器的应用用于分离密切相关的MIOX同类物.

主要成果:

基于ExuR的生物传感器在受控条件下对葡萄糖呈现出理想的反应.
当应用于不同的MIOX同类物时,生物传感器性能偏离,表明系统可变性.
电路架构和培养条件的修改成功减少了变异.
优化的生物传感器被有效地用于分离两个密切相关的MIOX同类.

结论:

基于ExuR的生物传感器可以适应和优化,用于选MIOX变种.
对生物传感器设计和培养条件的修改对于复杂的生物应用中强大的性能至关重要.
这项工作证明了生物传感器在酶工程和同源蛋白质分离中的实用性.