相关实验视频

Updated: Jul 26, 2025

Identification of protein complexes with quantitative proteomics in S. cerevisiae
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Identification of protein complexes with quantitative proteomics in S. cerevisiae

Published on: March 4, 2009

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在酵母S. cerevisiae中的两个核孔复合异型的差异亲和力净化和质谱分析

Pierre Bensidoun1, Daniel Zenklusen2, Marlene Oeffinger3

  • 1Institut de Recherches Cliniques de Montréal, 110 Avenue des Pins Ouest, Montréal, QC H2W 1R7, Canada; Département de Biochimie et Médecine Moléculaire, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, Canada.

STAR protocols
|June 16, 2023
PubMed
概括

研究人员开发了一种方法来隔离和分析酵母菌中两个核孔综合体 (NPC) 类型. 这种技术有助于理解细胞核内的NPC功能和蛋白质相互作用.

关键词:
细胞生物学 细胞生物学质谱测量质量谱测量分子生物学分子生物学

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Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
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