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Published on: November 12, 2012

阶段分离招募聚合诱导的排放素为快速E. 大肠杆菌检测检测

Syed Maricar¹, Sushanth Gudlur¹, Ali Miserez¹

¹Biological and Biomimetic Material Laboratory (BBML), Center for Sustainable Materials (SusMat), School of Materials Science and Engineering, Nanyang Technological University (NTU), Singapore 637553, Singapore.

Analytical chemistry

|June 16, 2023

概括

这项研究引入了一种新的生物传感器,用于快速E. 使用自组装凝析物和聚合诱导排放 (AIE) - 素检测大肠杆菌. 该测试提供了一种比目前用于识别细菌污染的方法更快的替代方法.

科学领域:

生物分子工程是生物分子工程.
生物感应技术技术的生物感应技术
微生物学微生物学

背景情况:

生物分子凝结物主要用于药物输送,因为它具有高效的分子捕获.
它们在生物传感中的应用在很大程度上仍未被探索.
快速和灵敏地检测像E. coli这样的细菌. 大肠杆菌对公共健康至关重要.

研究的目的:

开发一种简单,快速和灵敏的检测方法来检测E. 大肠杆菌.
探索分相缩物在生物传感中的使用.
为了利用聚合诱导排放 (AIE) 素进行视觉检测.

主要方法:

设计的相分离凝析物包含一个E. 大肠杆菌外膜蛋白酶 (OmpT) 识别站点.
在缩物中招募了AIE-素,在聚合时光.
利用OmpT的酶活性来分裂,破坏凝结物形成和灭光.

主要成果:

使用重组酶并随后与E.用E.成功检测OmpT活性. 大肠杆菌K-12.
取得了 10⁸ CFU/mL E.E. 的检测. 在2小时内,在含有刺的水样本中检测出大肠杆菌K-12.
在培养前阶段显示出检测1-10 CFU/mL的潜力,比商业套件快得多.

结论:

开发的测定方法提供了一种快速和可视检测的E.E.方法. 大肠杆菌的识别.
测试平台可适应检测其他阴性细菌和诊断相关的蛋白酶.
进一步优化序列可以提高检测极限和检测速度.

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