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相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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A Microfluidic Technique to Probe Cell Deformability
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基于尺寸和刚度的多阶段微流体细胞分类方法和芯片.

Gaolin Li1, Yuan Ji2, Yihui Wu2

  • 1Changchun Institute of Optics, Fine Mechanics and Physics (CIOMP), Chinese Academy of Sciences, Changchun, China; University of Chinese Academy of Sciences, Beijing, China.

Biosensors & bioelectronics
|June 16, 2023
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概括
此摘要是机器生成的。

这项研究引入了一种新型的多阶段微流体芯片,用于从血液中高纯度分类循环瘤细胞 (CTC). 该方法结合了基于尺寸和基于刚度的分类,以实现高效且无标签的CTC隔离和分析.

关键词:
细胞分类 细胞分类形通道芯片 形通道芯片确定性的横向移位.微流体芯片是一个微流体.

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科学领域:

  • 生物医学工程 生物医学工程
  • 微流体学 微流体学
  • 癌症的诊断 癌症的诊断

背景情况:

  • 循环瘤细胞 (CTC) 的高性能分类对于液体活检至关重要.
  • 传统的基于大小的方法,如确定性横向位移 (DLD),当细胞大小差异最小时,其特异性较低.
  • CTCs的硬度低于白细胞,提供了一个替代的分类参数.

研究的目的:

  • 开发一个多阶段的微流体系统,用于CTC的无标签,高纯度和高通量分类和分析.
  • 通过整合基于尺寸和基于刚度的分离技术来提高CTC分类的特异性.
  • 通过优化微柱设计,提高DLD分类中的流体调节.

主要方法:

  • 一个带有滴状微柱 (DMC) 的双阵列DLD芯片被设计用于初始基于大小的CTC分类.
  • 一个基于刚性的形通道芯片被开发出来,以净化CTC从污染白细胞中.
  • 拉曼光谱法用于无标签的细胞类型识别.
  • 四个DMC DLD芯片并行进行高通量样本处理.

主要成果:

  • 优化的DLD芯片实现了2.5毫升/分钟的样本处理速度,回收率为96.30 ± 2.10%,纯度为98.25 ± 2.48%.
  • 形通道芯片通过根据硬度差异捕获白细胞,提高了CTC纯度的1.8倍.
  • 综合系统提供了高效的,无标签的CTC隔离和分析.

结论:

  • 开发的多阶段微流体系统为CTC隔离和分析提供了高效和特定的方法.
  • 使用DMC优化的DLD芯片显著提高了流体调节和排序性能.
  • 结合基于尺寸和刚度的分类,为具有挑战性的CTC分离场景提供了强大的方法.