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谷氨酸在早期断奶期间促进肠干细胞介导的小肠上皮细胞发育:参与WNT信号传递

Junquan Tian¹, Yuying Li², Xuetai Bao²

¹National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-ecological Processes in Subtropical Region, Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan 410125, China; College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410000, China; University of Chinese Academy of Sciences, Beijing 100008, China.

Stem cell reports

|June 16, 2023

概括

谷氨酸 (Gln) 支持肠干细胞 (ISC) 活动,并在早期断奶后促进上皮细胞再生. 这突出了 Glnn.

科学领域:

胃肠道学和发育生物学
营养科学营养科学
干细胞生物学干细胞生物学

背景情况:

早期断奶会破坏小肠上皮的发育,增加胃肠道疾病的风险.
血和牛奶中丰富的谷氨胺 (Gln) 已知有助于肠道健康.
在断奶后,Gln在调节肠干细胞 (ISC) 活动中的特定作用仍然在很大程度上未被描述.

研究的目的:

调查谷氨酸 (Gln) 对肠干细胞 (ISC) 活性和上皮细胞再生在早期断奶的背景下的影响.
阐明 Gln 影响 ISC 功能的潜在分子机制.

主要方法:

利用早期断奶小鼠模型和体外肠道有机体培养.
评估了上皮形态,ISC增殖和再生能力.
通过基因表达分析和途径抑制,研究了WNT信号通路的参与.

主要成果:

谷氨酸 (Gln) 的使用缓解了早期断奶引起的上皮缩,并增强了ISC介导的上皮再生.
在实验室中,GLN剥夺损害了ISC介导的再生和密室裂变.
Gln剂量依赖增强了WNT信号,这对其对ISC活动的影响至关重要,因为WNT信号阻塞取消了Gln的好处.

结论:

谷氨酸 (Gln) 在促进肠上皮的发育和早期断奶后的修复方面发挥着关键作用.
Gln通过增强WNT信号来加速干细胞介导的肠道再生.
这些发现为 Gln 如何促进维持肠道健康提供了新的机制性见解.

关键词:

在WNT信号传输中.早期的断奶早期的断奶谷氨胺是一种肠道上皮质的发展.肠道干细胞是什么?肠道干细胞是什么?

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