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一种活体减弱的DIVA疫苗提供了对Listeria monocytogenes在绵羊中挑战的保护

Fanzeng Meng¹, Tengfei Zhu¹, Chao Chen¹

¹Jiangsu Key Laboratory of Zoonosis, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People 's Republic of China, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, China.

Microbial pathogenesis

|June 16, 2023

概括

一种新型Listeria monocytogenes (Lm) 候选疫苗在绵羊中证明了安全性和有效性,为致命挑战提供了78%的保护. 这种疫苗还可以区分感染者和接种疫苗的动物 (DIVA),这对于疾病控制至关重要.

科学领域:

兽医免疫学兽医免疫学
食品传播病原体研究
疫苗的研发工作正在进行中.

背景情况:

李斯特菌单细胞菌 (Lm) 是一种危险的食物传染病原体.
血清型4b Lm导致大多数人类和动物的李斯特菌病爆发.
需要有效的疫苗来控制牲畜中的LM感染.

研究的目的:

评估Lm血清型4b新型候选疫苗 (NTSNΔactA/plcB/orfX) 在绵羊中的安全性,免疫性和保护功效.
评估疫苗在区分感染者和接种疫苗的动物 (DIVA) 中的潜力.

主要方法:

一种三重基因删除菌株 (NTSNΔactA/plcB/orfX) 被开发为疫苗候选物.
绵羊接种疫苗,并通过感染动态,临床症状和病理学来评估安全性.
通过测量幽默性免疫反应来评估免疫性.
通过将接种疫苗的绵羊与致命的野生型Lm菌株进行挑战来确定保护性疗效.
通过血清检测针对listeriolysin O (LLO) 和胺固醇特异性脂酶C (PI-PLC) 的抗体来评估DIVA的能力.

主要成果:

该NTSNΔactA/plcB/orfX菌株在绵羊中显示出足够的安全性.
候选疫苗显著刺激了幽默性免疫反应.
接种疫苗的羊对致命的野生类型Lm挑战表现出78%的免疫保护.
该疫苗通过LLO和PI-PLC抗体的血清学测试证明了DIVA的潜力.

结论:

血清型4b Lm候选疫苗在绵羊中是安全的,免疫的和有效的.
该疫苗具有DIVA特征,可以区分受感染和接种疫苗的动物.
这种疫苗有望预防绵羊和潜在的其他牲畜和家禽的LM感染.

关键词:

迪瓦迪瓦迪瓦迪瓦幽默反应的反应免疫保护免疫保护免疫保护李斯特里亚单细胞原生菌 (Listeria monocytogenes) 是一种这是活体减弱疫苗.羊的李斯特菌病是羊的李斯特菌病.

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