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Updated: Jul 26, 2025

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甲状腺刺激激素-甲状腺激素信号传递通过抑制斑马鱼的时钟2/npas2促进昼夜调节

Cheng Ji¹, Yue Ou¹, Wangjianfei Yu²

¹Center for Circadian Clocks, Soochow University, Suzhou, Jiangsu 215123, China; School of Biology and Basic Medical Sciences, Suzhou Medical College, Soochow University, Suzhou, Jiangsu 215123, China.

Journal of genetics and genomics = Yi chuan xue bao

|June 16, 2023

概括

昼夜时钟直接调节斑马鱼的甲状腺刺激激素 (TSH) 生产,影响生长和新陈代谢. 这项研究揭示了TSH-甲状腺激素信号传递.

科学领域:

内分泌学在内分泌学.
时间生物学时间生物学
斑马鱼模型的模型

背景情况:

甲状腺刺激激素 (TSH) 对甲状腺功能,发育和新陈代谢至关重要.
先天性甲状腺功能低下症 (CH) 源于TSH生产缺陷,导致发育问题.
TSH昼夜节律的分子基础及其与昼夜时钟的相互作用尚未完全理解.

研究的目的:

研究斑马鱼中TSH昼夜调节的分子机制.
阐明TSH-甲状腺激素 (TH) 在昼夜时钟中信号传递的作用.
为了描述斑马鱼TSHβ亚单元 (tshba) 基因的功能.

主要方法:

对幼虫和成年斑马鱼的基因表达和蛋白质节律性的分析.
斑马鱼的生长和特征tshba淘汰突变 (tshba-/-).
斑马鱼的运动运动活动监测和转录组分析.

主要成果:

斑马鱼的tsba,TSH,甲状腺素 (T4) 和三甲状腺素 (T3) 都表现出每天的节律性.
循环时钟通过E-box和D-box元素直接调节shba表达.
tshba-/-突变体显示CH,其特征是T4/T3水平低和生长迟缓.
tshba 干扰改变了运动活动节奏和核心时钟和下丘脑-垂体-甲状腺 (HPT) 轴基因的表达.
TSH-TH信号传递通过甲状腺反应元件 (TRE) 影响时钟2/npas2表达.

结论:

斑马鱼tsheba是生物钟的直接目标.
TSH-TH信号传递是斑马鱼昼夜调节的组成部分.
干扰TSH - 昼夜时钟轴导致先天性甲状腺功能低下症和节律变化.

关键词:

循环节律是循环节律的节奏.这就是TALEN.甲状腺激素激素是甲状腺激素的一种.甲状腺刺激激素子单元βa (tshba)斑马鱼是一种斑马鱼.

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