电容合的无接触导电检测,以考虑液体色谱中的系统诱导的梯度变形
Leon E Niezen1, Tijmen S Bos2, Peter J Schoenmakers1
1Analytical-Chemistry Group, van 't Hoff Institute for Molecular Sciences, Faculty of Science, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands; Centre for Analytical Sciences Amsterdam (CASA), the Netherlands.
Analytica chimica acta
|June 16, 2023
概括
准确预测分析物保留在梯度流液色谱 (LC) 需要纠正系统诱导的梯度变形. 电容合无接触导电检测 (C4D) 测量了实际的梯度配置文件,提高了方法的可转移性.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
背景情况:
- 在渐变流液色谱 (LC) 中的方法开发可能是耗时的.
- 经验建模预测了分析物保留,但受到系统诱导的梯度变形的阻碍,特别是在的梯度中.
- 准确的预测和方法转移需要对仪器特定的梯度变形进行校正.
研究的目的:
- 开发一种方法来测量LC中的实际梯度配置文件.
- 为了研究梯度变形对保留模型的影响.
- 提高LC保留模型的系统间可转移性.
主要方法:
- 使用电容合"无接触"导电检测 (C4D) 进行梯度配置测量.
- C4D具有较低的检测体积 (~0.05μL) 和高压兼容性 (>80 MPa).
- 测量了各种各样的溶剂梯度 (例如,水-乙二,水-甲醇) 没有标记物,证明了一种通用方法.
主要成果:
- 发现每个溶剂组合,流量和梯度持续时间的梯度配置是独特的.
- 通过将编程的梯度与两个分布函数的加权和合并来准确地描述测量的配置文件.
- 对测量渐变形状的校正显著改善了各种分析物 (例如,多,炭,聚乙烯标准) 的保留模型的系统间可转移性.
结论:
- 电容合无接触导电检测 (C4D) 是用于测量实际LC梯度配置的多功能工具.
- 准确的梯度分析对于可靠的保留建模和不同LC系统之间的成功方法转移至关重要.
- 这种方法提高了在梯度偏移LC中的实证建模的可预测性和适用性.
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