不同代谢途径对工程化Corynebacterium glutamicum中的伊塔康酸生产的影响
Taghreed Elkasaby1, Dao Duy Hanh2, Hideo Kawaguchi2
1Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan; Botany Department, Faculty of Science, Mansoura University, 60 Elgomhoria St, Mansoura 35516, Egypt.
Journal of bioscience and bioengineering
|June 16, 2023
概括
改造的Corynebacterium glutamicum可以通过cis或trans通道产生伊塔康酸 (IA). 使用Ustilago maydis基因的跨途径产生了更高的IA标位,这表明它对生物基生产的优越性.
科学领域:
- 生物技术和代谢工程 生物技术和代谢工程
- 工业微生物学 工业微生物学
- 合成生物学 合成生物学
背景情况:
- 伊塔科尼酸 (IA) 是高分子的有价值的生物基建构块.
- 目前的IA生产通常依赖于Aspergillus terreus cadA基因的异质表达.
- 工程化Corynebacterium glutamicum为替代IA生产途径提供了一个平台.
研究的目的:
- 通过两种不同的途径,对Corynebacterium glutamicum进行工程设计,用于生产 itaconic acid (IA).
- 为了比较哺乳动物cis-pathway (Irg1) 与真菌trans-pathway (Adi1/Tad1) 的有效性,用于IA生物合成.
- 为了评估IA从各种碳来源的生产性能,使用工程C. glutamicum.
主要方法:
- 在C. glutamicum中,Mus musculus Irg1基因 (cis路径) 的异质表达.
- 在C. glutamicum中Ustilago maydis Adi1和Tad1基因 (跨通路) 的异质表达.
- 使用葡萄糖,麦芽糖和糖作为碳来源的工程菌株的料批发发酵.
主要成果:
- 两种 cis 和 trans 途径都使得工程 C. glutamicum 中的 IA 生产成为可能.
- 跨通路菌株 (C. glutamicum pCH-Tad1optadi1opt) 获得了更高的IA标位:12.25 g/L (葡萄糖),11.34 g/L (马尔托斯) 和11.02 g/L (糖糖).
- 跨路径的摩尔产量达到了0.43mol/mol的糖糖.
结论:
- 使用Ustilago maydis Adi1和Tad1基因的跨通路在工程Corynebacterium glutamicum中更有效地生产 itaconic acid.
- 这项研究表明了C. glutamicum作为生物基伊塔康酸通过替代途径生产的主机的潜力.
- 跨路径为工业IA生物合成中常用的cis路径提供了一个有希望的替代方案.
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