mTORC1/rpS6和mTORC2/PKC通过Eriocheir sinensis中的Arp3介导的actin微纤维组织来调节精子生成
Zhen-Fang Li1, Hong-Yu Qi1, Jia-Ming Wang1
1The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
Cell and tissue research
|June 16, 2023
概括
哺乳动物的拉巴胺素 (mTOR) 途径的标调节了甲类动物的精子生成. 这项研究表明mTORC1/rpS6和mTORC2/PKC信号传导通过actin组织影响精子发育和丸屏障完整性.
科学领域:
- 生殖生物学 生殖生物学
- 细胞信号传递 细胞信号传递
- 甲动物研究研究
背景情况:
- 猛素 (mTOR) 途径的哺乳动物点对细胞功能至关重要,包括哺乳动物精子生成.
- 它在甲动物繁殖中的作用在很大程度上尚未被探索.
- mTOR通过两个复合体发挥作用:mTOR复合体1 (mTORC1) 和mTOR复合体2 (mTORC2).
研究的目的:
- 研究mTORC1和mTORC2信号在Eriocheir sinensis的精子生成中的功能.
- 阐明潜在的分子机制,特别是关于丸屏障完整性和行为组织.
主要方法:
- 下游效应者的克隆:mTORC1的核糖体蛋白S6 (rpS6) 和mTORC2.2的蛋白激酶C (PKC).
- 使用敲除rpS6/PKC和使用mTOR抑制剂Torin治疗的功能分析1.1.
- 评估精子生成,丸屏障完整性,结合蛋白和丝状动蛋白 (F-actin) 组织.
主要成果:
- 阻断rpS6/PKC或Torin1治疗导致精子生成缺陷,包括生殖细胞损失和精子保留.
- 丸屏障完整性被破坏,结 protein 的表达变化.
- 这些干扰与F-actin网络的混乱有关,由与actin相关的蛋白3 (Arp3) 介导.
结论:
- 在E. sinensis中,mTORC1/rpS6和mTORC2/PKC通路对于精子生成至关重要.
- 这些通路通过Arp3-介导的活性组织来调节精子发育和丸屏障功能.
- 这项研究揭示了对无脊椎动物繁殖中的mTOR信号传递的新见解.
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