巨型囊泡的动力放松验证了二元脂质混合物中的扩散软化
Kayla Sapp1, Mina Aleksanyan2,3, Kaitlyn Kerr1
1Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, 20892 Maryland, USA.
Physical review. E
|June 17, 2023
概括
对于细胞功能至关重要的膜硬度,使用巨型单状囊泡波动来测量. 发现脂质的混合物使膜变软,影响囊泡动力学.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 材料科学 材料科学 材料科学
背景情况:
- 生物膜硬度会影响细胞机械对囊泡的工作.
- 模型膜硬度是由巨型单囊 (GUV) 表面波动决定的.
- 脂质组成和曲率灵敏度会影响波纹和放松动态.
研究的目的:
- 为了研究脂质组成和膜硬性之间的关系.
- 为了验证影响膜柔软性的分子机制.
- 了解脂质扩散如何影响膜波动的放松.
主要方法:
- 使用相对比显微镜观察GUV表面波纹.
- 在由酸丁胆-酸丁乙醇胺混合物组成的GUV中进行波纹的动态分析.
- 将多元组件与单元膜的波浪分布进行比较.
主要成果:
- 侧面构成波动与多元组件膜的表面波动相结合.
- 观察到波纹的分布更广泛,受脂质扩散的影响.
- 酸丁胆-酸丁乙醇胺混合物导致膜比单组分膜软25%.
结论:
- 该研究验证了一种分子机制,该机制有助于降低脂质混合物中的膜硬度.
- 这种机制与生物膜中发现的多样化和曲率敏感的脂质有关.
- 了解膜力学是涉及脂质形状变化的细胞过程的关键.
更多相关视频
09:29Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
Published on: January 19, 2020
8.5K
10:08Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
Published on: October 24, 2017
9.3K
相关概念视频
Atomic Nuclei: Types of Nuclear Relaxation
333
Nuclear relaxation restores the equilibrium population imbalance and can occur via spin–lattice or spin–spin mechanisms, which are first-order exponential decay processes.
In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers...
In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers...
333
Protein Diffusion in the Membrane
4.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.4K
